Production of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentation

Panagiotou, G; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:27Z
dc.date.available	2014-03-01T01:19:27Z
dc.date.issued	2003	en
dc.identifier.issn	0926-6690	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15502
dc.subject	Cellulases	en
dc.subject	Corn stover	en
dc.subject	Fusarium oxysporum	en
dc.subject	Solid state culture	en
dc.subject	Xylanases	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Agronomy	en
dc.subject.other	Bioreactors	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	pH effects	en
dc.subject.other	Corn stover	en
dc.subject.other	Crops	en
dc.subject.other	enzyme	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Zea mays	en
dc.title	Production of cellulolytic and xylanolytic enzymes by Fusarium oxysporum grown on corn stover in solid state fermentation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0926-6690(03)00018-9	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0926-6690(03)00018-9	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Corn stover is an abundant, potential fermentation substrate. Production of cellulolytic and xylanolytic enzymes by the mesophilic fungus Fusarium oxysporum under solid state culture (SSC) on corn stover was enhanced by optimization of the type of nitrogen source, initial moisture level, growth temperature and initial pH of the culture medium. Under these conditions, yields as high as 304, 4.1, 0.140, 1840 and 0.041 U/g of carbon source of endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase and beta-xylosidase, respectively, were obtained. SCC in a laboratory horizontal bioreactor using the optimized medium allowed the large scale production of the multienzymic system in similar yields. Chromogenic (fluorogenic) 4-methylumbelliferyl beta-glycosides of cellobiose and xylobiose were used to characterize the major activities of the multienzyme component, after separation by isoelectric focusing (IEF) electrophoresis. The zymograms indicated one major cellulase and four xylanase activities exhibiting pI values 5 and 5, 6, 7.3, 8.3, respectively. (C) 2003 Elsevier Science B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Industrial Crops and Products	en
dc.identifier.doi	10.1016/S0926-6690(03)00018-9	en
dc.identifier.isi	ISI:000183480200005	en
dc.identifier.volume	18	en
dc.identifier.issue	1	en
dc.identifier.spage	37	en
dc.identifier.epage	45	en