Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures

Topakas, E; Stamatis, H; Biely, P; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Biely, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:27Z
dc.date.available	2014-03-01T01:19:27Z
dc.date.issued	2003	en
dc.identifier.issn	0168-1656	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15508
dc.subject	Esterification	en
dc.subject	Feruloyl esterase	en
dc.subject	Fusarium oxysporum	en
dc.subject	Microemulsions	en
dc.subject	Purification	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Catalysts	en
dc.subject.other	Column chromatography	en
dc.subject.other	Esterification	en
dc.subject.other	Microemulsions	en
dc.subject.other	Organic solvents	en
dc.subject.other	pH effects	en
dc.subject.other	Synthesis (chemical)	en
dc.subject.other	Molecular mass	en
dc.subject.other	Biotechnology	en
dc.subject.other	4 nitrophenyl 2 o feruloyl alpha levo arabinofuranoside	en
dc.subject.other	4 nitrophenyl 5 o feruloyl alpha levo arabinofuranoside	en
dc.subject.other	ester derivative	en
dc.subject.other	ferulic acid	en
dc.subject.other	feruloylesterase	en
dc.subject.other	fungal enzyme	en
dc.subject.other	furan derivative	en
dc.subject.other	isoenzyme	en
dc.subject.other	methylcaffeate	en
dc.subject.other	methylcoumarate	en
dc.subject.other	methylferulate	en
dc.subject.other	methylsinapate	en
dc.subject.other	organic solvent	en
dc.subject.other	phenol derivative	en
dc.subject.other	sepharose	en
dc.subject.other	unclassified drug	en
dc.subject.other	water	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	article	en
dc.subject.other	bran	en
dc.subject.other	column chromatography	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme assay	en
dc.subject.other	enzyme kinetics	en
dc.subject.other	enzyme purification	en
dc.subject.other	enzyme stability	en
dc.subject.other	esterification	en
dc.subject.other	fungus culture	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	hydrolysis	en
dc.subject.other	incubation time	en
dc.subject.other	molecular weight	en
dc.subject.other	nonhuman	en
dc.subject.other	optical rotation	en
dc.subject.other	pH	en
dc.subject.other	priority journal	en
dc.subject.other	protein interaction	en
dc.subject.other	Sporothrix	en
dc.subject.other	sporotrichum thermophile	en
dc.subject.other	temperature	en
dc.subject.other	thermostability	en
dc.subject.other	Arabinose	en
dc.subject.other	Carboxylic Ester Hydrolases	en
dc.subject.other	Catalysis	en
dc.subject.other	Chromatography, Agarose	en
dc.subject.other	Cinnamates	en
dc.subject.other	Coenzymes	en
dc.subject.other	Coumaric Acids	en
dc.subject.other	Emulsions	en
dc.subject.other	Enzyme Activation	en
dc.subject.other	Enzyme Stability	en
dc.subject.other	Esterification	en
dc.subject.other	Fusarium	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Hydroxybenzoic Acids	en
dc.subject.other	Molecular Weight	en
dc.subject.other	Organic Chemicals	en
dc.subject.other	Solvents	en
dc.subject.other	Species Specificity	en
dc.subject.other	Sporothrix	en
dc.subject.other	Substrate Specificity	en
dc.subject.other	Temperature	en
dc.subject.other	Water	en
dc.subject.other	Xylosidases	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Triticum aestivum	en
dc.title	Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0168-1656(02)00363-2	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0168-1656(02)00363-2	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	An extracellular feruloyl esterase (FAE-II) from the culture filtrates of Fusarium oxysporum F3 was purified to homogeneity by SP-Sepharose, t-butyl-HIC and Sephacryl S-200 column chromatography. The protein corresponded to molecular mass and pI values of 27 kDa and 9.9, respectively. The enzyme was optimally active at pH 7 and 45 degreesC. The purified esterase was fully stable at pH 7.0-9.0 and temperature up to 45 degreesC after 1 h incubation. Determination of k(cat)/K-m revealed that the enzyme hydrolysed methyl sinapinate 6, 21 and 40 times more efficiently than methyl ferulate, methyl coumarate and methyl caffeate, respectively. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 but inactive to the C-2 positions of arabinofuranose such as 4-nitrophenyl 5-O-transferuloyl-alpha-L-arabinofuranoside and 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside. In the presence of Sporotrichum thermophile xylanase, there was a significant release of ferulic acid from destarched wheat bran by FAE-II, indicating a synergistic interaction between FAE-II and S. thermophile xylanase. FAE-II by itself could release only little ferulic acid from destarched wheat bran. The potential of FAE-II for the synthesis of various phenolic acid esters was tested using as a reaction system a surfactantless microemulsion formed in ternary mixture consisting of n-hexane, 1-propanol and water. (C) 2003 Elsevier Science B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Biotechnology	en
dc.identifier.doi	10.1016/S0168-1656(02)00363-2	en
dc.identifier.isi	ISI:000182299400004	en
dc.identifier.volume	102	en
dc.identifier.issue	1	en
dc.identifier.spage	33	en
dc.identifier.epage	44	en