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Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures

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dc.contributor.author Topakas, E en
dc.contributor.author Stamatis, H en
dc.contributor.author Biely, P en
dc.contributor.author Kekos, D en
dc.contributor.author Macris, BJ en
dc.contributor.author Christakopoulos, P en
dc.date.accessioned 2014-03-01T01:19:27Z
dc.date.available 2014-03-01T01:19:27Z
dc.date.issued 2003 en
dc.identifier.issn 0168-1656 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/15508
dc.subject Esterification en
dc.subject Feruloyl esterase en
dc.subject Fusarium oxysporum en
dc.subject Microemulsions en
dc.subject Purification en
dc.subject.classification Biotechnology & Applied Microbiology en
dc.subject.other Catalysts en
dc.subject.other Column chromatography en
dc.subject.other Esterification en
dc.subject.other Microemulsions en
dc.subject.other Organic solvents en
dc.subject.other pH effects en
dc.subject.other Synthesis (chemical) en
dc.subject.other Molecular mass en
dc.subject.other Biotechnology en
dc.subject.other 4 nitrophenyl 2 o feruloyl alpha levo arabinofuranoside en
dc.subject.other 4 nitrophenyl 5 o feruloyl alpha levo arabinofuranoside en
dc.subject.other ester derivative en
dc.subject.other ferulic acid en
dc.subject.other feruloylesterase en
dc.subject.other fungal enzyme en
dc.subject.other furan derivative en
dc.subject.other isoenzyme en
dc.subject.other methylcaffeate en
dc.subject.other methylcoumarate en
dc.subject.other methylferulate en
dc.subject.other methylsinapate en
dc.subject.other organic solvent en
dc.subject.other phenol derivative en
dc.subject.other sepharose en
dc.subject.other unclassified drug en
dc.subject.other water en
dc.subject.other xylan endo 1,3 beta xylosidase en
dc.subject.other article en
dc.subject.other bran en
dc.subject.other column chromatography en
dc.subject.other enzyme activity en
dc.subject.other enzyme assay en
dc.subject.other enzyme kinetics en
dc.subject.other enzyme purification en
dc.subject.other enzyme stability en
dc.subject.other esterification en
dc.subject.other fungus culture en
dc.subject.other Fusarium oxysporum en
dc.subject.other hydrolysis en
dc.subject.other incubation time en
dc.subject.other molecular weight en
dc.subject.other nonhuman en
dc.subject.other optical rotation en
dc.subject.other pH en
dc.subject.other priority journal en
dc.subject.other protein interaction en
dc.subject.other Sporothrix en
dc.subject.other sporotrichum thermophile en
dc.subject.other temperature en
dc.subject.other thermostability en
dc.subject.other Arabinose en
dc.subject.other Carboxylic Ester Hydrolases en
dc.subject.other Catalysis en
dc.subject.other Chromatography, Agarose en
dc.subject.other Cinnamates en
dc.subject.other Coenzymes en
dc.subject.other Coumaric Acids en
dc.subject.other Emulsions en
dc.subject.other Enzyme Activation en
dc.subject.other Enzyme Stability en
dc.subject.other Esterification en
dc.subject.other Fusarium en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Hydroxybenzoic Acids en
dc.subject.other Molecular Weight en
dc.subject.other Organic Chemicals en
dc.subject.other Solvents en
dc.subject.other Species Specificity en
dc.subject.other Sporothrix en
dc.subject.other Substrate Specificity en
dc.subject.other Temperature en
dc.subject.other Water en
dc.subject.other Xylosidases en
dc.subject.other Corynascus heterothallicus en
dc.subject.other Fusarium en
dc.subject.other Fusarium oxysporum en
dc.subject.other Sporotrichum en
dc.subject.other Triticum aestivum en
dc.title Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures en
heal.type journalArticle en
heal.identifier.primary 10.1016/S0168-1656(02)00363-2 en
heal.identifier.secondary http://dx.doi.org/10.1016/S0168-1656(02)00363-2 en
heal.language English en
heal.publicationDate 2003 en
heal.abstract An extracellular feruloyl esterase (FAE-II) from the culture filtrates of Fusarium oxysporum F3 was purified to homogeneity by SP-Sepharose, t-butyl-HIC and Sephacryl S-200 column chromatography. The protein corresponded to molecular mass and pI values of 27 kDa and 9.9, respectively. The enzyme was optimally active at pH 7 and 45 degreesC. The purified esterase was fully stable at pH 7.0-9.0 and temperature up to 45 degreesC after 1 h incubation. Determination of k(cat)/K-m revealed that the enzyme hydrolysed methyl sinapinate 6, 21 and 40 times more efficiently than methyl ferulate, methyl coumarate and methyl caffeate, respectively. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 but inactive to the C-2 positions of arabinofuranose such as 4-nitrophenyl 5-O-transferuloyl-alpha-L-arabinofuranoside and 4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside. In the presence of Sporotrichum thermophile xylanase, there was a significant release of ferulic acid from destarched wheat bran by FAE-II, indicating a synergistic interaction between FAE-II and S. thermophile xylanase. FAE-II by itself could release only little ferulic acid from destarched wheat bran. The potential of FAE-II for the synthesis of various phenolic acid esters was tested using as a reaction system a surfactantless microemulsion formed in ternary mixture consisting of n-hexane, 1-propanol and water. (C) 2003 Elsevier Science B.V. All rights reserved. en
heal.publisher ELSEVIER SCIENCE BV en
heal.journalName Journal of Biotechnology en
dc.identifier.doi 10.1016/S0168-1656(02)00363-2 en
dc.identifier.isi ISI:000182299400004 en
dc.identifier.volume 102 en
dc.identifier.issue 1 en
dc.identifier.spage 33 en
dc.identifier.epage 44 en


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