Purification and characterization of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters

Topakas, E; Stamatis, H; Mastihubova, M; Biely, P; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Mastihubova, M	en
dc.contributor.author	Biely, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:28Z
dc.date.available	2014-03-01T01:19:28Z
dc.date.issued	2003	en
dc.identifier.issn	0141-0229	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15509
dc.subject	Feruloyl esterase	en
dc.subject	Fusarium oxysporum	en
dc.subject	Phenolic acid esters	en
dc.subject	Surfactantless microemulsions	en
dc.subject	Transesterification	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Enzymes	en
dc.subject.other	Hydrophobicity	en
dc.subject.other	Ion exchange	en
dc.subject.other	pH effects	en
dc.subject.other	Hydrophobic interaction	en
dc.subject.other	Organic compounds	en
dc.subject.other	4 nitrophenyl 2 o feruloyl alpha levo arabinofuranoside	en
dc.subject.other	arabinofuranosidase	en
dc.subject.other	butanol	en
dc.subject.other	caffeic acid	en
dc.subject.other	ester derivative	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid	en
dc.subject.other	ferulic acid methyl ester	en
dc.subject.other	feruloyl esterase	en
dc.subject.other	fungal enzyme	en
dc.subject.other	hexane	en
dc.subject.other	para coumaric acid	en
dc.subject.other	sinapic acid	en
dc.subject.other	unclassified drug	en
dc.subject.other	water	en
dc.subject.other	article	en
dc.subject.other	catalysis	en
dc.subject.other	chromatography	en
dc.subject.other	enzyme release	en
dc.subject.other	fungus culture	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	gel filtration chromatography	en
dc.subject.other	incubation time	en
dc.subject.other	ion exchange chromatography	en
dc.subject.other	microemulsion	en
dc.subject.other	molecular weight	en
dc.subject.other	nonhuman	en
dc.subject.other	pH	en
dc.subject.other	Sporothrix	en
dc.subject.other	temperature	en
dc.subject.other	transesterification	en
dc.subject.other	wheat bran	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Felis catus	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Triticum aestivum	en
dc.title	Purification and characterization of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0141-0229(03)00213-8	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0141-0229(03)00213-8	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	An extracellular feruloyl esterase (FoFAE-I) from the culture filtrates of Fusarium oxysporum F3 was purified to homogeneity by ion-exchange, hydrophobic interaction and gel filtration chromatographies. The protein corresponded to molecular mass and pI values of 31 kDa and 9.5, respectively. The enzyme was optimally active at pH 7.0 and 55 degreesC. The purified esterase was fully stable at pH 7.0-9.0 and temperature up to 30 degreesC. Determination of k(cat)/K-m revealed that the enzyme hydrolysed methyl p-coumarate (MpCA) 4.5, 9, and 239 times more efficiently than methyl caffeate (MCA), methyl ferulate (MFA) and methyl sinapinate (MSA), respectively. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose but showed preference for the ester at position 2. 4-Nitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside (NPh-5-Fe-Araf) was hydrolysed 100 times more efficiently than 4-nitrophenyl-5-O-trans-feruloyl-alpha-L-arabinofuranoside (NPh-2-Fe-Araf). Ferulic acid (FA) was efficiently released from destarched wheat bran (DSWB) when the esterase was incubated together with xylanase from Sporotrichum thermophile (a maximum of 92% total ferulic acid released after 4 h incubation). FoFAE-I by itself could release FA but at a level almost five-fold lower than that obtained in the presence of xylanase. The potential of FAE-I for the synthesis of various phenolic acid esters was tested using as a reaction system a surfactantless microemulsions formed in ternary mixture consisting of n-hexane, 1-butanol and water. (C) 2003 Elsevier Inc. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	Enzyme and Microbial Technology	en
dc.identifier.doi	10.1016/S0141-0229(03)00213-8	en
dc.identifier.isi	ISI:000185366500025	en
dc.identifier.volume	33	en
dc.identifier.issue	5	en
dc.identifier.spage	729	en
dc.identifier.epage	737	en