Biosynthesis of fructo-oligosaccharides by Sporotrichum thermophile during submerged batch cultivation in high sucrose media

Katapodis, P; Kalogeris, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:19:58Z
dc.date.available	2014-03-01T01:19:58Z
dc.date.issued	2004	en
dc.identifier.issn	0175-7598	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15786
dc.subject	Acid Hydrolysis	en
dc.subject	Compatible Solute	en
dc.subject	Fatty Acid	en
dc.subject	glutamic acid	en
dc.subject	Membrane Fluidity	en
dc.subject	Osmotic Pressure	en
dc.subject	Size Exclusion Chromatography	en
dc.subject	Amino Acid	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	1 kestose	en
dc.subject.other	6 kestose	en
dc.subject.other	alanine	en
dc.subject.other	amino acid	en
dc.subject.other	arginine	en
dc.subject.other	carbohydrate derivative	en
dc.subject.other	fructose oligosaccharide	en
dc.subject.other	glutamic acid	en
dc.subject.other	leucine	en
dc.subject.other	lysine	en
dc.subject.other	neokestose	en
dc.subject.other	sucrose	en
dc.subject.other	unclassified drug	en
dc.subject.other	biotechnology	en
dc.subject.other	fungus	en
dc.subject.other	sucrose	en
dc.subject.other	article	en
dc.subject.other	biosynthesis	en
dc.subject.other	concentration response	en
dc.subject.other	culture medium	en
dc.subject.other	fungus growth	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	hydrolysis	en
dc.subject.other	nonhuman	en
dc.subject.other	osmosis	en
dc.subject.other	osmotic pressure	en
dc.subject.other	solute	en
dc.subject.other	Sporothrix	en
dc.subject.other	sporotrichum thermophile	en
dc.subject.other	Amino Acids	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Cell Membrane	en
dc.subject.other	Chromatography, High Pressure Liquid	en
dc.subject.other	Culture Media	en
dc.subject.other	Cytoplasm	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Membrane Fluidity	en
dc.subject.other	Membrane Lipids	en
dc.subject.other	Oligosaccharides	en
dc.subject.other	Osmotic Pressure	en
dc.subject.other	Sporothrix	en
dc.subject.other	Sucrose	en
dc.subject.other	Trisaccharides	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Fungi	en
dc.subject.other	Sporothrix	en
dc.subject.other	Sporotrichum	en
dc.title	Biosynthesis of fructo-oligosaccharides by Sporotrichum thermophile during submerged batch cultivation in high sucrose media	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00253-003-1348-x	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00253-003-1348-x	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	Biosynthesis of fructo-oligosaccharides (FOS) was observed during growth of the thermophilic fungus Sporotrichum thermophile on media containing high sucrose concentrations. Submerged batch cultivation with the optimum initial sucrose concentration of 250 g/l allowed the production of 12.5 g FOS/l. The FOS mixture obtained was composed of three sugars, which were isolated by size-exclusion chromatography. They were characterized by acid hydrolysis and HPLC as 1-kestose, 6-kestose and neokestose. The mechanism of osmotic adaptation of S. thermophile was investigated and sugars and amino acids were found to be the predominant compatible solutes. The fungus accumulated glutamic acid, arginine, alanine, leucine and lysine, in order to balance the outer osmotic pressure. Fatty acid analysis of the membrane lipids showed a relatively high percentage of unsaturated lipids, which is known to be associated with high membrane fluidity.	en
heal.publisher	SPRINGER-VERLAG	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s00253-003-1348-x	en
dc.identifier.isi	ISI:000188112900006	en
dc.identifier.volume	63	en
dc.identifier.issue	4	en
dc.identifier.spage	378	en
dc.identifier.epage	382	en