Induction and partial characterization of intracellular β-fructofuranosidase from Thermoascus aurantiacus and its application in the synthesis of 6-kestose

Katapodis, P; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:20:37Z
dc.date.available	2014-03-01T01:20:37Z
dc.date.issued	2004	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/15989
dc.subject	β-Fructofuranosidase	en
dc.subject	6-Kestose	en
dc.subject	Thermoascus aurantiacus	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Biocatalysts	en
dc.subject.other	Bioreactors	en
dc.subject.other	Carbon	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Cultivation	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Optimization	en
dc.subject.other	β-fructofuranosodase	en
dc.subject.other	Batch cultivation	en
dc.subject.other	Carbon source	en
dc.subject.other	Thermophilic fungus	en
dc.subject.other	Fungi	en
dc.subject.other	Catalysts	en
dc.subject.other	Concentration	en
dc.subject.other	Cultivation	en
dc.subject.other	Enzymes	en
dc.subject.other	Fungi	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Optimization	en
dc.subject.other	Reactors	en
dc.subject.other	Fungi	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Induction and partial characterization of intracellular β-fructofuranosidase from Thermoascus aurantiacus and its application in the synthesis of 6-kestose	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11274-004-0684-z	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s11274-004-0684-z	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	Production of beta-fructofuranosidase from the thermophilic fungus Thermoascus aurantiacus was enhanced by optimization of the type of nitrogen source as well as the type and concentration of carbon source. Submerged batch cultivation in a laboratory bioreactor (7 l) using the optimized medium allowed the production of 85 mU/ml of beta-fructofuranosidase. The enzyme showed both transfructosylating and hydrolytic activities and was optimally active at 60 degreesC and pH 5.0. The enzyme showed the ability to catalyse the synthesis of 1-kestose and the reaction was maximized at 30% (w/v) initial sucrose concentration.	en
heal.publisher	KLUWER ACADEMIC PUBL	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s11274-004-0684-z	en
dc.identifier.isi	ISI:000225186800003	en
dc.identifier.volume	20	en
dc.identifier.issue	7	en
dc.identifier.spage	667	en
dc.identifier.epage	672	en