NADPH-dependent D-aldose reductases and xylose fermentation in Fusarium oxysporum

Panagiotou, G; Christakopoulos, P

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:21:06Z
dc.date.available	2014-03-01T01:21:06Z
dc.date.issued	2004	en
dc.identifier.issn	1389-1723	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16070
dc.subject	Acetoin	en
dc.subject	Fusarium oxysporum	en
dc.subject	Xylose	en
dc.subject	Xylose reductases	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Food Science & Technology	en
dc.subject.other	Electrons	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Metabolism	en
dc.subject.other	Batch cultivation	en
dc.subject.other	Stirred tank reactors	en
dc.subject.other	Enzymes	en
dc.subject.other	acetoin	en
dc.subject.other	alcohol	en
dc.subject.other	aldehyde reductase	en
dc.subject.other	reduced nicotinamide adenine dinucleotide	en
dc.subject.other	reduced nicotinamide adenine dinucleotide phosphate	en
dc.subject.other	xylitol	en
dc.subject.other	xylose	en
dc.subject.other	aeration	en
dc.subject.other	aerobic fermentation	en
dc.subject.other	anaerobic fermentation	en
dc.subject.other	article	en
dc.subject.other	batch fermentation	en
dc.subject.other	controlled study	en
dc.subject.other	enzyme metabolism	en
dc.subject.other	enzyme purification	en
dc.subject.other	enzyme subunit	en
dc.subject.other	fungal strain	en
dc.subject.other	fungus culture	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	nonhuman	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.title	NADPH-dependent D-aldose reductases and xylose fermentation in Fusarium oxysporum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S1389-1723(04)70209-1	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S1389-1723(04)70209-1	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	Two aldose (xylose) reductases (ARI and ARII) from Fusarium oxysporum were purified and characterized. The native ARI was a monomer with M-r 41000, pI 5.2 and showed a 52-fold preference for NADPH over NADH, while ARII was homodimeric with a subunit of M-r 37000, pI 3.6 and a 60-fold preference for NADPH over NADH. In this study, the influence of aeration and the response to the addition of electron acceptors on xylose fermentation by F. oxysporum were also studied. The batch cultivation of F. oxysporum on xylose was performed under aerobic, anaerobic and oxygen-limited conditions in stirred tank reactors. Oxygen limitation had considerable influence on xylose metabolism. Under anaerobic conditions (0 vvm), xylitol was the main product with a maximum yield of 0.34 mole of xylitol/mole of xylose while the maximum ethanol yield (1.02 moles of ethanol/mole of xylose) was obtained under aerobic conditions (0.3 vvm). When the artificial electron acceptor acetoin was added to an anaerobic batch fermentation of xylose by F. oxysporum, the ethanol yield increased while xylitol excretion was also decreased.	en
heal.publisher	SOC BIOSCIENCE BIOENGINEERING JAPAN	en
heal.journalName	Journal of Bioscience and Bioengineering	en
dc.identifier.doi	10.1016/S1389-1723(04)70209-1	en
dc.identifier.isi	ISI:000222580200003	en
dc.identifier.volume	97	en
dc.identifier.issue	5	en
dc.identifier.spage	299	en
dc.identifier.epage	304	en