Production and partial characterization of alkaline feruloyl esterases by Fusarium oxysporum during submerged batch cultivation

Topakas, E; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:21:15Z
dc.date.available	2014-03-01T01:21:15Z
dc.date.issued	2004	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16164
dc.subject	Alkaline feruloyl esterase	en
dc.subject	Fusarium oxysporum	en
dc.subject	Zymogram	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Zea mays	en
dc.title	Production and partial characterization of alkaline feruloyl esterases by Fusarium oxysporum during submerged batch cultivation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/B:WIBI.0000023830.38119.3f	en
heal.identifier.secondary	http://dx.doi.org/10.1023/B:WIBI.0000023830.38119.3f	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	Production of feruloyl esterases (FAEs) by Fusarium oxysporum was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source. Submerged batch cultivation in a laboratory bioreactor (17 1) produced activity at 82 nkat g(-1) dry substrate (corn cobs) which compared favorably to those reported for the other microorganisms. Use of de-esterified corn cobs as carbon source decreased FAE production by 5.5-fold compared to untreated corn cobs even though ferulic acid (FA) was added to the concentration found in alkali-extracts of corn cobs. Production of FAE does not therefore, require FA, however, production is diminished by the removal of esterified FA from the growth substrate. Optimal FAE activity was observed at pH 7 and 50 degreesC with 68 and 55% activity at pH 8 and pH 9, respectively. The esterase was fully stable at pH 5-8 and up to 40 degreesC and retained 72 and 40% of its activity after 6 h at pH 9 and pH 10, respectively. After separation by isoelectric focusing electrophoresis, a zymogram indicated one major FAE activity exhibiting pI value of 10.5.	en
heal.publisher	KLUWER ACADEMIC PUBL	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1023/B:WIBI.0000023830.38119.3f	en
dc.identifier.isi	ISI:000221544200005	en
dc.identifier.volume	20	en
dc.identifier.issue	3	en
dc.identifier.spage	245	en
dc.identifier.epage	250	en