Production of phenolics from corn cobs by coupling enzymic treatment and solid state fermentation

Topakas, E; Kalogeris, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:21:15Z
dc.date.available	2014-03-01T01:21:15Z
dc.date.issued	2004	en
dc.identifier.issn	1618-0240	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16165
dc.subject	Enzyme	en
dc.subject	Solid State Fermentation	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	cinnamic acid	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid	en
dc.subject.other	para coumaric acid	en
dc.subject.other	phenol derivative	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	enzyme activity	en
dc.subject.other	fermentation	en
dc.subject.other	phenolic compound	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	corn	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme assay	en
dc.subject.other	enzyme substrate	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	extraction	en
dc.subject.other	fungus growth	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	hydrolysis	en
dc.subject.other	nonhuman	en
dc.subject.other	solid state fermentation	en
dc.subject.other	Sporothrix	en
dc.subject.other	Fungi	en
dc.subject.other	Sporothrix	en
dc.subject.other	Zea mays	en
dc.title	Production of phenolics from corn cobs by coupling enzymic treatment and solid state fermentation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elsc.200420025	en
heal.identifier.secondary	http://dx.doi.org/10.1002/elsc.200420025	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	A two-stage process that combined solid-state fermentation (SSF) and subsequent enzymic treatment was used in order to release p-coumaric (p-CA) and ferulic acid (FA) from corn cobs. Sporotrichum thermophile was grown on corn cobs under SSF conditions, and the production of cinnamoyl esterases and xylanases was studied over 7 days. The time course of enzyme production showed a maximum activity of 1483 nkat/g, 0.3 nkat/g and 0.067 nkat/g for xylanase, feruloyl esterase, and p-coumaroyl esterase, respectively. The importance of the moisture level of the growth substrate was discussed. After SSF, the fermented substrate was directly exposed to autohydrolysis and the in situ produced multienzyme system was successfully used for the partial degradation of cell wall components and the liberation of p-CA and FA. A yield of 0.85 g/kg and 0.38 g/kg FA and p-CA, respectively, of dry matter of corn cobs was obtained.	en
heal.publisher	WILEY-V C H VERLAG GMBH	en
heal.journalName	Engineering in Life Sciences	en
dc.identifier.doi	10.1002/elsc.200420025	en
dc.identifier.isi	ISI:000223597100012	en
dc.identifier.volume	4	en
dc.identifier.issue	3	en
dc.identifier.spage	283	en
dc.identifier.epage	286	en