Separation of organoselenium compounds and their electrochemical detection

Ochsenkuhn-Petropoulou, MTh; Tsopelas, FN

dc.contributor.author	Ochsenkuhn-Petropoulou, MTh	en
dc.contributor.author	Tsopelas, FN	en
dc.date.accessioned	2014-03-01T01:21:22Z
dc.date.available	2014-03-01T01:21:22Z
dc.date.issued	2004	en
dc.identifier.issn	1618-2642	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16214
dc.subject	Ion-exchange separation	en
dc.subject	Organoselenium compounds	en
dc.subject	Selenocystamine	en
dc.subject	Selenourea	en
dc.subject	Urine	en
dc.subject	Voltammetry	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Amines	en
dc.subject.other	Ammonium compounds	en
dc.subject.other	Body fluids	en
dc.subject.other	Electrochemical electrodes	en
dc.subject.other	Electrochemistry	en
dc.subject.other	Ethanol	en
dc.subject.other	Hydrochloric acid	en
dc.subject.other	Ion exchange	en
dc.subject.other	Mass spectrometry	en
dc.subject.other	Polypropylenes	en
dc.subject.other	Positive ions	en
dc.subject.other	Selenium compounds	en
dc.subject.other	Separation	en
dc.subject.other	Toxicity	en
dc.subject.other	Urea	en
dc.subject.other	Ion exchange separation	en
dc.subject.other	Organoselenium compounds	en
dc.subject.other	Selenocystamine	en
dc.subject.other	Selenourea	en
dc.subject.other	Urine	en
dc.subject.other	Voltammetry	en
dc.subject.other	Organometallics	en
dc.subject.other	carbon	en
dc.subject.other	cystamine	en
dc.subject.other	drug derivative	en
dc.subject.other	electrolyte	en
dc.subject.other	organoselenium derivative	en
dc.subject.other	selenocystamine	en
dc.subject.other	selenourea	en
dc.subject.other	urea	en
dc.subject.other	adult	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	electrochemistry	en
dc.subject.other	fly ash	en
dc.subject.other	human	en
dc.subject.other	isolation and purification	en
dc.subject.other	male	en
dc.subject.other	particulate matter	en
dc.subject.other	sensitivity and specificity	en
dc.subject.other	soil	en
dc.subject.other	time	en
dc.subject.other	urinalysis	en
dc.subject.other	urine	en
dc.subject.other	Adult	en
dc.subject.other	Carbon	en
dc.subject.other	Cystamine	en
dc.subject.other	Electrochemistry	en
dc.subject.other	Electrolytes	en
dc.subject.other	Humans	en
dc.subject.other	Male	en
dc.subject.other	Organoselenium Compounds	en
dc.subject.other	Particulate Matter	en
dc.subject.other	Sensitivity and Specificity	en
dc.subject.other	Soil	en
dc.subject.other	Time Factors	en
dc.subject.other	Urea	en
dc.subject.other	Urinalysis	en
dc.subject.other	Urine	en
dc.title	Separation of organoselenium compounds and their electrochemical detection	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00216-004-2511-7	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00216-004-2511-7	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	A simplified procedure based on ion-exchange separation of selenourea (Se-U) and selenocystamine (Se-CM), which have very close half-wave potential when they are simultaneously analyzed by voltammetric techniques, has been developed and optimized. Thus, selenocystamine remains in the cation exchanger Purolite C 100 H, whereas selenourea is found in the effluent and is determined by square wave cathodic stripping voltammetry using Na2CO3 as electrolyte. Selenocystamine is then eluted from the cation exchanger using 4 M HCl and analyzed by square wave cathodic stripping voltammetry in the HCl solution. For each voltammetric determination the corresponding parameters were investigated and optimized; the obtained detection limits were 0.3 ng Se mL -1 for Se-CM and 2 ng Se mL-1 for Se-U. A flow sheet for the separation of inorganic (Se(IV) and Se(VI)) and organoselenium compounds (Se-U, Se-CM, (CH3)2Se2, and (CH 3)2Se) developed for their electrochemical detection is presented and it was successfully applied to a certified reference material, an environmental soil sample, and a urine sample. © Springer-Verlag 2004.	en
heal.publisher	SPRINGER HEIDELBERG	en
heal.journalName	Analytical and Bioanalytical Chemistry	en
dc.identifier.doi	10.1007/s00216-004-2511-7	en
dc.identifier.isi	ISI:000223474200006	en
dc.identifier.volume	379	en
dc.identifier.issue	5-6	en
dc.identifier.spage	770	en
dc.identifier.epage	776	en