Studies on a N-acetyl-β-D-glucosaminidase produced by Fusarium oxysporum F3 grown in solid-state fermentation

Gkargkas, K; Mamma, D; Nedev, G; Topakas, E; Christakopoulos, P; Kekos, D; Macris, BJ

dc.contributor.author	Gkargkas, K	en
dc.contributor.author	Mamma, D	en
dc.contributor.author	Nedev, G	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:21:28Z
dc.date.available	2014-03-01T01:21:28Z
dc.date.issued	2004	en
dc.identifier.issn	0032-9592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16252
dc.subject	Fusarium oxysporum	en
dc.subject	N-Acetyl-β-D-glucosaminidase	en
dc.subject	Production	en
dc.subject	Purification	en
dc.subject	Solid-state fermentation	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Engineering, Chemical	en
dc.subject.other	Cell culture	en
dc.subject.other	Chromatographic analysis	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	Fungi	en
dc.subject.other	Ion exchange	en
dc.subject.other	Enzyme yield	en
dc.subject.other	Solid-state fermentation	en
dc.subject.other	Organic compounds	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Triticum aestivum	en
dc.title	Studies on a N-acetyl-β-D-glucosaminidase produced by Fusarium oxysporum F3 grown in solid-state fermentation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0032-9592(03)00287-5	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0032-9592(03)00287-5	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	Fusarium oxysporum F3 produced N-acetyl-beta-D-glucosaminidase when grown on wheat bran and chitin as carbon sources in solid-state fermentation. The initial moisture content and pH of growth medium were 65% and 6.0, respectively, and the enzyme yield 23.6 U g(-1) carbon source. Two isozymes of N-acetyl-beta-D-glucosaminidase, called N-acetyl-beta-D-glucosaminidases I and II, were isolated from the culture filtrate of F. oxysporum F3. The filtrate was subjected to ammonium sulphate fractionation followed by anion exchange, get filtration, hydrophobic interaction and cation exchange chromatography. The optimum pH of isozymes I and II was 5.0 and 6.0, respectively, whereas maximum activity of both isozymes was obtained at 40degreesC. The K-m of isozymes I and II was 49.6 and 48.6 muM and the V-max 1.24 and 0.26 mu mol mg(-1) min(-1), respectively, on p-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate. The molecular mass of isozymes I and II was calculated to be 67 kDa by SDS-PAGE. (C) 2003 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Process Biochemistry	en
dc.identifier.doi	10.1016/S0032-9592(03)00287-5	en
dc.identifier.isi	ISI:000223089500038	en
dc.identifier.volume	39	en
dc.identifier.issue	11	en
dc.identifier.spage	1599	en
dc.identifier.epage	1605	en