Synergy between enzymes involved in the degradation of insoluble wheat flour arabinoxylan

Vardakou, M; Katapodis, P; Topakas, E; Kekos, D; Macris, BJ; Christakopoulos, P

dc.contributor.author	Vardakou, M	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:21:35Z
dc.date.available	2014-03-01T01:21:35Z
dc.date.issued	2004	en
dc.identifier.issn	14668564	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16265
dc.subject	Feruloyl-oligosaccharides	en
dc.subject	Hydrolysis	en
dc.subject	S. thermophile	en
dc.subject	Size-exclusion chromatography (SEC)	en
dc.subject.other	arabinoxylan	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid	en
dc.subject.other	oligosaccharide	en
dc.subject.other	unclassified drug	en
dc.subject.other	xylan	en
dc.subject.other	xylan 1,4 beta xylosidase	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	xylan endo 1,4 beta xylosidase	en
dc.subject.other	anion exchange chromatography	en
dc.subject.other	article	en
dc.subject.other	calculation	en
dc.subject.other	carbohydrate analysis	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	controlled study	en
dc.subject.other	degradation kinetics	en
dc.subject.other	enzyme degradation	en
dc.subject.other	fluor	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	incubation time	en
dc.subject.other	mass spectrometry	en
dc.subject.other	mathematical model	en
dc.subject.other	molecular weight	en
dc.subject.other	protein hydrolysis	en
dc.subject.other	reversed phase high performance liquid chromatography	en
dc.subject.other	spectrophotometry	en
dc.subject.other	Sporothrix	en
dc.subject.other	Thermoascus	en
dc.subject.other	wheat	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.subject.other	Triticum aestivum	en
dc.title	Synergy between enzymes involved in the degradation of insoluble wheat flour arabinoxylan	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S1466-8564(03)00044-4	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S1466-8564(03)00044-4	en
heal.publicationDate	2004	en
heal.abstract	Two microbial endo-β-1,4-xylanases (EXs, EC 3.2.1.8) belonging to glycanase families 10 and 11 were examined for their ability to release ferulic acid (FA) from water-unextractable arabinoxylan (WU-AX) in the presence of a feruloyl esterase (FoFAE-II) from Fusarium oxysporum. WU-AX was incubated with different levels of a Thermoascus aurantiacus family 10 (XYLI) and a Sporotrichum thermophile family 11 (XYLA) endoxylanases. At 10 g/l arabinoxylan, enzyme concentrations (KE values) needed to obtain half-maximal hydrolysis rates for FA release were 0.18 and 0.44 nM for the xylanases from T. aurantiacus and S. thermophile, respectively. Determination of Vmax/KE revealed that the family 10 enzyme performed 4.3 times more efficiently than the family 11 enzyme in liberation of FA when a feruloyl esterase is present. Molecular weights of the products formed were assessed and separation of feruloyl-oligosaccharides was achieved by anion-exchange and size-exclusion chromatography (SEC). The results showed that the degradation of the xylan backbone was influenced strongest by the action of xylanases while the presence of the esterase mainly resulted in the release of ferulic acid from the produced short chain feruloylated xylo-oligosaccharides by the action of xylanases. © 2003 Elsevier Ltd. All rights reserved.	en
heal.journalName	Innovative Food Science and Emerging Technologies	en
dc.identifier.doi	10.1016/S1466-8564(03)00044-4	en
dc.identifier.volume	5	en
dc.identifier.issue	1	en
dc.identifier.spage	107	en
dc.identifier.epage	112	en