A confocal microscopy study of the very early cellular response to oxidative stress induced by zinc phthalocyanine sensitization

Alexandratou, E; Yova, D; Loukas, S

dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T01:21:42Z
dc.date.available	2014-03-01T01:21:42Z
dc.date.issued	2005	en
dc.identifier.issn	0891-5849	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16329
dc.subject	Confocal microscopy	en
dc.subject	Oxidative stress	en
dc.subject	Phthalocyanine	en
dc.subject	Single cell level	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Endocrinology & Metabolism	en
dc.subject.other	calcium	en
dc.subject.other	phthalocyanine zinc	en
dc.subject.other	reactive oxygen metabolite	en
dc.subject.other	animal cell	en
dc.subject.other	apoptosis	en
dc.subject.other	article	en
dc.subject.other	cell level	en
dc.subject.other	cell membrane potential	en
dc.subject.other	cell pH	en
dc.subject.other	cellular distribution	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	confocal microscopy	en
dc.subject.other	fetus	en
dc.subject.other	fluorescence	en
dc.subject.other	human	en
dc.subject.other	image analysis	en
dc.subject.other	image processing	en
dc.subject.other	laser	en
dc.subject.other	lens	en
dc.subject.other	mitochondrial membrane	en
dc.subject.other	necrosis	en
dc.subject.other	nonhuman	en
dc.subject.other	oxidative stress	en
dc.subject.other	pathology	en
dc.subject.other	photoactivation	en
dc.subject.other	priority journal	en
dc.subject.other	protein targeting	en
dc.subject.other	real time echography	en
dc.subject.other	Calcium	en
dc.subject.other	Cells, Cultured	en
dc.subject.other	Fibroblasts	en
dc.subject.other	Humans	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Indoles	en
dc.subject.other	Membrane Potentials	en
dc.subject.other	Microscopy, Confocal	en
dc.subject.other	Mitochondria	en
dc.subject.other	Mitochondrial Membranes	en
dc.subject.other	Organometallic Compounds	en
dc.subject.other	Oxidative Stress	en
dc.subject.other	Photosensitizing Agents	en
dc.subject.other	Reactive Oxygen Species	en
dc.title	A confocal microscopy study of the very early cellular response to oxidative stress induced by zinc phthalocyanine sensitization	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.freeradbiomed.2005.06.006	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.freeradbiomed.2005.06.006	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	Oxidative stress has been involved in several biological and pathological processes. Reactive oxygen species have been shown to play both beneficial and deleterious roles. The present work contributes to the understanding of the very early events of cellular response to oxidative stress. Oxidative stress was produced intracellularly by light activation of zinc phthalocyanine (ZnPc) at a light dose that did not lead to apoptosis or necrosis. Phthalocyanine was photoactivated using the 647-nm laser line of a confocal microscope through the objective lens causing oxidative stress and allowing observation of the evoked phenomena at the single cell level and in real time. Mitochondria membrane potential (Delta psi(m)), intracellular pH, calcium concentration, and generation of reactive oxygen species (ROS) were recorded using specific vital fluorescent probes and quantified by image processing and analysis. Subcellular localization of ZnPc was also studied in order to determine the primary and intermediate ROS target. (c) 2005 Elsevier Inc. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	Free Radical Biology and Medicine	en
dc.identifier.doi	10.1016/j.freeradbiomed.2005.06.006	en
dc.identifier.isi	ISI:000232870700001	en
dc.identifier.volume	39	en
dc.identifier.issue	9	en
dc.identifier.spage	1119	en
dc.identifier.epage	1127	en