Comparison of ultra-violet and inductively coupled plasma-atomic emission spectrometry for the on-line quantification of selenium species after their separation by reversed-phase liquid chromatography

Tsopelas, FN; Ochsenkuhn-Petropoulou, MTh; Mergias, IG; Tsakanika, LV

dc.contributor.author	Tsopelas, FN	en
dc.contributor.author	Ochsenkuhn-Petropoulou, MTh	en
dc.contributor.author	Mergias, IG	en
dc.contributor.author	Tsakanika, LV	en
dc.date.accessioned	2014-03-01T01:22:00Z
dc.date.available	2014-03-01T01:22:00Z
dc.date.issued	2005	en
dc.identifier.issn	0003-2670	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16435
dc.subject	Inductively coupled plasma-atomic emission spectrometry	en
dc.subject	Reversed-phase liquid chromatography	en
dc.subject	Selenium speciation	en
dc.subject	Selenized yeast	en
dc.subject	Selenomethionine	en
dc.subject	Ultra-violet spectrometry	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Emission spectroscopy	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Enzymes	en
dc.subject.other	Inductively coupled plasma	en
dc.subject.other	Liquid chromatography	en
dc.subject.other	Selenium	en
dc.subject.other	Ultraviolet radiation	en
dc.subject.other	Yeast	en
dc.subject.other	Atomic emission spectrometry (AES)	en
dc.subject.other	n-octanesulfonic acid	en
dc.subject.other	Selenites	en
dc.subject.other	UV spectrometry	en
dc.subject.other	Separation	en
dc.subject.other	cystamine derivative	en
dc.subject.other	selenate	en
dc.subject.other	selenite	en
dc.subject.other	selenium	en
dc.subject.other	selenocystine	en
dc.subject.other	selenomethionine	en
dc.subject.other	sodium	en
dc.subject.other	sulfonic acid derivative	en
dc.subject.other	accuracy	en
dc.subject.other	article	en
dc.subject.other	atomic emission spectrometry	en
dc.subject.other	chromatography	en
dc.subject.other	comparative study	en
dc.subject.other	laboratory	en
dc.subject.other	priority journal	en
dc.subject.other	quantitative analysis	en
dc.subject.other	reversed phase liquid chromatography	en
dc.subject.other	separation technique	en
dc.subject.other	spectral sensitivity	en
dc.subject.other	ultraviolet spectroscopy	en
dc.subject.other	Viola (angiosperm)	en
dc.title	Comparison of ultra-violet and inductively coupled plasma-atomic emission spectrometry for the on-line quantification of selenium species after their separation by reversed-phase liquid chromatography	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.aca.2005.03.021	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.aca.2005.03.021	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	An analytical approach for selenium speciation using liquid chromatography (LC) coupled with ultra-violet (UV) and inductively coupled plasma-atomic emission spectrometry (ICP-AES) was developed. The separation of the investigated selenium species, selenites, selenates, selenomethionine, selenocystine, selenocystamine and dimethyldiselenide was accomplished in less than 6 min on a BIO Wide Pore RP-18 column using sodium salt of n-octanesulfonic acid as ion-pairing modifier. The on-line detection of the separated selenium species was performed using UV spectrometry at the optimum wavelength of 192 nm, obtained by the UV spectra of the investigated individual selenium species. ICP-AES was also used as element specific on-line detector, after its coupling with the chromatographic system. The UV and ICP-AES detectors were compared for their suitability, including sensitivity and detection limits, for the on-line quantification of the six selenium species. The developed LC-UV as well as LC-ICP-AES techniques were successfully applied to a selenized yeast candidate reference material, after its enzymatic extraction with protease XIV It was found that the described LC-UV technique is suitable for the determination of selenomethionine, the main selenium compound in the yeast, with an accuracy of 5%, although the UV detector is not element specific and it is rarely used for selenium speciation. This finding can prove valuable for routine laboratories to perform selenium speciation in such materials. (c) 2005 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Analytica Chimica Acta	en
dc.identifier.doi	10.1016/j.aca.2005.03.021	en
dc.identifier.isi	ISI:000229175100046	en
dc.identifier.volume	539	en
dc.identifier.issue	1-2	en
dc.identifier.spage	327	en
dc.identifier.epage	333	en