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Comparison of ultra-violet and inductively coupled plasma-atomic emission spectrometry for the on-line quantification of selenium species after their separation by reversed-phase liquid chromatography

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dc.contributor.author Tsopelas, FN en
dc.contributor.author Ochsenkuhn-Petropoulou, MTh en
dc.contributor.author Mergias, IG en
dc.contributor.author Tsakanika, LV en
dc.date.accessioned 2014-03-01T01:22:00Z
dc.date.available 2014-03-01T01:22:00Z
dc.date.issued 2005 en
dc.identifier.issn 0003-2670 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/16435
dc.subject Inductively coupled plasma-atomic emission spectrometry en
dc.subject Reversed-phase liquid chromatography en
dc.subject Selenium speciation en
dc.subject Selenized yeast en
dc.subject Selenomethionine en
dc.subject Ultra-violet spectrometry en
dc.subject.classification Chemistry, Analytical en
dc.subject.other Emission spectroscopy en
dc.subject.other Enzyme kinetics en
dc.subject.other Enzymes en
dc.subject.other Inductively coupled plasma en
dc.subject.other Liquid chromatography en
dc.subject.other Selenium en
dc.subject.other Ultraviolet radiation en
dc.subject.other Yeast en
dc.subject.other Atomic emission spectrometry (AES) en
dc.subject.other n-octanesulfonic acid en
dc.subject.other Selenites en
dc.subject.other UV spectrometry en
dc.subject.other Separation en
dc.subject.other cystamine derivative en
dc.subject.other selenate en
dc.subject.other selenite en
dc.subject.other selenium en
dc.subject.other selenocystine en
dc.subject.other selenomethionine en
dc.subject.other sodium en
dc.subject.other sulfonic acid derivative en
dc.subject.other accuracy en
dc.subject.other article en
dc.subject.other atomic emission spectrometry en
dc.subject.other chromatography en
dc.subject.other comparative study en
dc.subject.other laboratory en
dc.subject.other priority journal en
dc.subject.other quantitative analysis en
dc.subject.other reversed phase liquid chromatography en
dc.subject.other separation technique en
dc.subject.other spectral sensitivity en
dc.subject.other ultraviolet spectroscopy en
dc.subject.other Viola (angiosperm) en
dc.title Comparison of ultra-violet and inductively coupled plasma-atomic emission spectrometry for the on-line quantification of selenium species after their separation by reversed-phase liquid chromatography en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.aca.2005.03.021 en
heal.identifier.secondary http://dx.doi.org/10.1016/j.aca.2005.03.021 en
heal.language English en
heal.publicationDate 2005 en
heal.abstract An analytical approach for selenium speciation using liquid chromatography (LC) coupled with ultra-violet (UV) and inductively coupled plasma-atomic emission spectrometry (ICP-AES) was developed. The separation of the investigated selenium species, selenites, selenates, selenomethionine, selenocystine, selenocystamine and dimethyldiselenide was accomplished in less than 6 min on a BIO Wide Pore RP-18 column using sodium salt of n-octanesulfonic acid as ion-pairing modifier. The on-line detection of the separated selenium species was performed using UV spectrometry at the optimum wavelength of 192 nm, obtained by the UV spectra of the investigated individual selenium species. ICP-AES was also used as element specific on-line detector, after its coupling with the chromatographic system. The UV and ICP-AES detectors were compared for their suitability, including sensitivity and detection limits, for the on-line quantification of the six selenium species. The developed LC-UV as well as LC-ICP-AES techniques were successfully applied to a selenized yeast candidate reference material, after its enzymatic extraction with protease XIV It was found that the described LC-UV technique is suitable for the determination of selenomethionine, the main selenium compound in the yeast, with an accuracy of 5%, although the UV detector is not element specific and it is rarely used for selenium speciation. This finding can prove valuable for routine laboratories to perform selenium speciation in such materials. (c) 2005 Elsevier B.V. All rights reserved. en
heal.publisher ELSEVIER SCIENCE BV en
heal.journalName Analytica Chimica Acta en
dc.identifier.doi 10.1016/j.aca.2005.03.021 en
dc.identifier.isi ISI:000229175100046 en
dc.identifier.volume 539 en
dc.identifier.issue 1-2 en
dc.identifier.spage 327 en
dc.identifier.epage 333 en


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