Fermentation performance and intracellular metabolite profiling of Fusarium oxysporum cultivated on a glucose-xylose mixture

Panagiotou, G; Christakopoulos, P; Villas-Boas, SG; Olsson, L

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Villas-Boas, SG	en
dc.contributor.author	Olsson, L	en
dc.date.accessioned	2014-03-01T01:22:24Z
dc.date.available	2014-03-01T01:22:24Z
dc.date.issued	2005	en
dc.identifier.issn	0141-0229	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16551
dc.subject	Aeration	en
dc.subject	Ethanol	en
dc.subject	Fusarium oxysporum	en
dc.subject	Glucose/xylose mixture	en
dc.subject	Intracellular metabolites	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Batch cultivation	en
dc.subject.other	Co-metabolism	en
dc.subject.other	Pentose phosphate pathway (PPP)	en
dc.subject.other	Ethanol	en
dc.subject.other	Glucose	en
dc.subject.other	Metabolism	en
dc.subject.other	Mixtures	en
dc.subject.other	Water aeration	en
dc.subject.other	Xylose	en
dc.subject.other	Fermentation	en
dc.subject.other	glucose	en
dc.subject.other	xylose	en
dc.subject.other	aeration	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	electron transport	en
dc.subject.other	enzyme metabolism	en
dc.subject.other	fermentation	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	metabolite	en
dc.subject.other	nonhuman	en
dc.subject.other	reduction	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.title	Fermentation performance and intracellular metabolite profiling of Fusarium oxysporum cultivated on a glucose-xylose mixture	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.enzmictec.2004.07.009	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.enzmictec.2004.07.009	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	The influence of aeration on the fermentation of a mixture of glucose and xylose by the naturally xylose fermenting fungus Fusarium oxysporum was studied in batch cultivations. The aeration level had considerable influence on the co-metabolism of glucose and xylose. Under anaerobic conditions xylose consumption was limited and xylitol was the main product of xylose metabolism. When the artificial electron acceptor acetoin was added xylose consumption increased by 95% with a significant improvement of ethanol yield from 0.96 to 1.52 mol/mol, mainly due to a 72% reduction of xylitol excretion. The profile of the phosphorylated intermediates during the fermentation suggests that a high glycolytic flux occurred during the oxygen-limited conditions, characterized by high efflux of glyceraldehyde-3-P (G3P) and fructose-6-P (F6P) from Pentose Phosphate Pathway (PPP) to Embden-Meyerhof-Parnas Pathway (EMP) resulting in the higher ethanol production. An accumulation of sedoheptulose-7-P (S7P) was also observed indicating a block in the PPP which affects the production of NADPH and results in the production of acetate and the reduced xylose consumption. (C) 2004 Elsevier Inc. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	Enzyme and Microbial Technology	en
dc.identifier.doi	10.1016/j.enzmictec.2004.07.009	en
dc.identifier.isi	ISI:000226195800013	en
dc.identifier.volume	36	en
dc.identifier.issue	1	en
dc.identifier.spage	100	en
dc.identifier.epage	106	en