Intracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol

Panagiotou, G; Villas-Boas, SG; Christakopoulos, P; Nielsen, J; Olsson, L

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Villas-Boas, SG	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Nielsen, J	en
dc.contributor.author	Olsson, L	en
dc.date.accessioned	2014-03-01T01:22:32Z
dc.date.available	2014-03-01T01:22:32Z
dc.date.issued	2005	en
dc.identifier.issn	0168-1656	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16608
dc.subject	glucose cultivation	en
dc.subject	ethanol production	en
dc.subject	Fusarium oxysporum	en
dc.subject	aeration	en
dc.subject	intracellular metabolites	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	AMINO-ACID POOLS	en
dc.subject.other	BATCH CULTIVATIONS	en
dc.subject.other	ASPERGILLUS-NIGER	en
dc.subject.other	PURIFICATION	en
dc.subject.other	STRAIN	en
dc.subject.other	ENDO-1,4-BETA-D-GLUCANASE	en
dc.subject.other	FERMENTATION	en
dc.subject.other	CELLULOSE	en
dc.subject.other	EXTRACTS	en
dc.subject.other	XYLANASE	en
dc.title	Intracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jbiotec.2004.09.011	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.jbiotec.2004.09.011	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	The filamentous fungus Fusarium oxysporum is known for its ability to produce ethanol by simultaneous saccharification and fermentation (SSF) of cellulose. However, the conversion rate is low and significant amounts of acetic acid are produced as a by-product. In this study, the growth characteristics of E oxysporum were evaluated in a minimal medium using glucose as the sole carbon source in aerobic, anaerobic and oxygen-limited batch cultivations. Under aerobic conditions the maximum specific growth rate was found to be 0.043 h(-1), and the highest ethanol yield (1.66 mol/mol) was found under anaerobic conditions. During the different phases of the cultivations, the intracellular profiles were determined under aerobic and anaerobic conditions. The profiles of the phosphorylated intermediates indicated that there was a high glycolytic flux at anaerobic growth conditions, characterized by high efflux of glyceraldehyde-3-phosphate (G3P) and fructose-6-phosphate (F6P) from the pentose phosphate pathway (PPP) to the Embden-Meyerhof-Parnas (EMP) pathway, resulting in the highest ethanol production under these conditions. The amino acid profile clearly suggests that the TCA cycle was primarily active under aerobic cultivation. On the other hand, the presence of high levels of gamma-amino-n-butyric acid (GABA) under anaerobic conditions suggests a functional GABA bypass and a possible block in the TCA cycle at these conditions. (C) 2004 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	JOURNAL OF BIOTECHNOLOGY	en
dc.identifier.doi	10.1016/j.jbiotec.2004.09.011	en
dc.identifier.isi	ISI:000226459000010	en
dc.identifier.volume	115	en
dc.identifier.issue	4	en
dc.identifier.spage	425	en
dc.identifier.epage	434	en