Light-induced cell separation in a tailored optical landscape

Paterson, L; Papagiakoumou, E; Milne, G; Garcs-Chavez, V; Tatarkova, SA; Sibbett, W; Gunn-Moore, FJ; Bryant, PE; Riches, AC; Dholakia, K

dc.contributor.author	Paterson, L	en
dc.contributor.author	Papagiakoumou, E	en
dc.contributor.author	Milne, G	en
dc.contributor.author	Garcs-Chavez, V	en
dc.contributor.author	Tatarkova, SA	en
dc.contributor.author	Sibbett, W	en
dc.contributor.author	Gunn-Moore, FJ	en
dc.contributor.author	Bryant, PE	en
dc.contributor.author	Riches, AC	en
dc.contributor.author	Dholakia, K	en
dc.date.accessioned	2014-03-01T01:22:34Z
dc.date.available	2014-03-01T01:22:34Z
dc.date.issued	2005	en
dc.identifier.issn	0003-6951	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16617
dc.subject.classification	Physics, Applied	en
dc.subject.other	Antibodies	en
dc.subject.other	Cells	en
dc.subject.other	Polarization	en
dc.subject.other	Silica	en
dc.subject.other	Cell populations	en
dc.subject.other	Lymphocites	en
dc.subject.other	Optical sorting	en
dc.subject.other	Optical properties	en
dc.title	Light-induced cell separation in a tailored optical landscape	en
heal.type	journalArticle	en
heal.identifier.primary	10.1063/1.2045548	en
heal.identifier.secondary	http://dx.doi.org/10.1063/1.2045548	en
heal.identifier.secondary	123901	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	We demonstrate passive optical sorting of cell populations in the absence of any externally driven fluid flow. Specifically, we report the movement of erythrocytes and lymphocytes in an optical landscape, consisting of a circularly symmetric light pattern created by a Bessel light beam. These distinct cell populations move, spontaneously and differentially, across the underlying periodic optical landscape. Thus, we were able to separate lymphocytes from a mixed population of cells containing erythrocytes and then collect the lymphocytes in a microcapillary reservoir. We also demonstrate an enhanced form of this separation that exploits the polarizability of silica microspheres by attaching spheres coated with antibodies to cell surface markers to a subpopulation of lymphocytes. These techniques may be applied using standard laboratory apparatus. (c) 2005 American Institute of Physics.	en
heal.publisher	AMER INST PHYSICS	en
heal.journalName	Applied Physics Letters	en
dc.identifier.doi	10.1063/1.2045548	en
dc.identifier.isi	ISI:000231907200068	en
dc.identifier.volume	87	en
dc.identifier.issue	12	en
dc.identifier.spage	1	en
dc.identifier.epage	3	en