Simultaneous saccharification and fermentation of cellulose by Fusarium oxysporum F3 - Growth characteristics and metabolite profiling

Panagiotou, G; Christakopoulos, P; Olsson, L

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Olsson, L	en
dc.date.accessioned	2014-03-01T01:23:05Z
dc.date.available	2014-03-01T01:23:05Z
dc.date.issued	2005	en
dc.identifier.issn	0141-0229	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16799
dc.subject	Cellulose	en
dc.subject	Fusarium oxysporum	en
dc.subject	GABA shunt	en
dc.subject	Metabolite profiling	en
dc.subject	Simultaneous saccharification and fermentation	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Amino acids	en
dc.subject.other	Biosynthesis	en
dc.subject.other	Enzymes	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucose	en
dc.subject.other	Metabolites	en
dc.subject.other	Saccharification	en
dc.subject.other	Aerobic conditions	en
dc.subject.other	Krebs cycle	en
dc.subject.other	Pentose phosphate pathway (PPP)	en
dc.subject.other	Volumetric productivity	en
dc.subject.other	Cellulose	en
dc.subject.other	4 aminobutyric acid	en
dc.subject.other	alcohol	en
dc.subject.other	amino acid	en
dc.subject.other	cellobiose	en
dc.subject.other	cellulase	en
dc.subject.other	cellulose	en
dc.subject.other	glucose	en
dc.subject.other	glucose 1,6 bisphosphate	en
dc.subject.other	organic compound	en
dc.subject.other	pentose phosphate	en
dc.subject.other	phosphoglucomutase	en
dc.subject.other	aerobic fermentation	en
dc.subject.other	anaerobic fermentation	en
dc.subject.other	article	en
dc.subject.other	citric acid cycle	en
dc.subject.other	controlled study	en
dc.subject.other	enzyme activity	en
dc.subject.other	fermentation technique	en
dc.subject.other	fungus growth	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	glycolysis	en
dc.subject.other	growth rate	en
dc.subject.other	nonhuman	en
dc.subject.other	phosphorylation	en
dc.subject.other	saccharification	en
dc.subject.other	Fusarium	en
dc.subject.other	Fusarium oxysporum	en
dc.title	Simultaneous saccharification and fermentation of cellulose by Fusarium oxysporum F3 - Growth characteristics and metabolite profiling	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.enzmictec.2004.12.029	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.enzmictec.2004.12.029	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	The simultaneous saccharification and fermentation (SSF) of cellulose by Fusarium oxysporum was investigated in the present study. It was found that E oxysporum grow with a maximum specific growth rate of 0.023 h(-1) on cellulose at aerobic conditions and that it can produce ethanol with a volumetric productivity of 0.044 g/L/h and a yield of 0.35 g/g cellulose under anaerobic conditions. The cellulase system in F oxysporum is well balanced as no cellobiose accumulated. The profile of the phosphorylated intermediates from Pentose Phosphate Pathway (PPP), Embden-Meyerhof-Parnas Pathway (EMP) and the key intermediates of the glycolytic pathway as well as extracellular organic and amino acids were determined during the fermentation in order to investigate the potential metabolic bottlenecks of the process. The high levels of intracellular glucose-1,6-DP a metabolic downstream of phoshoglucomutase also indicates limiting activities of this enzyme and difficulty of glucose to be channelled into biosynthetic and glycolytic pathways. The presence of high levels of gamma-aminobutyrate (GABA) under anaerobic conditions suggests a functional GABA bypass and possible block in the Krebs cycle. (c) 2005 Elsevier Inc. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	Enzyme and Microbial Technology	en
dc.identifier.doi	10.1016/j.enzmictec.2004.12.029	en
dc.identifier.isi	ISI:000227781000011	en
dc.identifier.volume	36	en
dc.identifier.issue	5-6	en
dc.identifier.spage	693	en
dc.identifier.epage	699	en