Size characterization of manganese species from liver extracts using size exclusion chromatography inductively coupled plasma mass spectrometry

Quintana, M; Klouda, AD; Ochsenkuhn-Petropoulou, M; Michalke, B

dc.contributor.author	Quintana, M	en
dc.contributor.author	Klouda, AD	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.contributor.author	Michalke, B	en
dc.date.accessioned	2014-03-01T01:23:05Z
dc.date.available	2014-03-01T01:23:05Z
dc.date.issued	2005	en
dc.identifier.issn	0003-2670	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16801
dc.subject	Dynamic reaction cell	en
dc.subject	Inductively coupled plasma mass spectrometry (ICP-MS)	en
dc.subject	Liver extract	en
dc.subject	Mn	en
dc.subject	Size exclusion chromatography	en
dc.subject	Speciation	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Inductively coupled plasma	en
dc.subject.other	Mass spectrometry	en
dc.subject.other	Size exclusion chromatography	en
dc.subject.other	Dynamic reaction cells	en
dc.subject.other	Liver extract	en
dc.subject.other	Manganese species	en
dc.subject.other	Speciation	en
dc.subject.other	Manganese	en
dc.subject.other	liver extract	en
dc.subject.other	manganese	en
dc.subject.other	article	en
dc.subject.other	atmosphere	en
dc.subject.other	atomic emission spectrometry	en
dc.subject.other	calibration	en
dc.subject.other	controlled study	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	human	en
dc.subject.other	human cell	en
dc.subject.other	human tissue	en
dc.subject.other	molecular weight	en
dc.subject.other	priority journal	en
dc.subject.other	signal noise ratio	en
dc.title	Size characterization of manganese species from liver extracts using size exclusion chromatography inductively coupled plasma mass spectrometry	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.aca.2005.08.029	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.aca.2005.08.029	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	Increased Mn levels are known to damage the central nervous system, resulting in motoric abnormalities and psychic disorder and finally resulting even in symptoms similar to Parkinson's disease. Monomethyl-Mn-pentadienyl-tricarbonyl is used as anti-knock agent and consequently Mn compounds are exhausted into air from automobiles. With additional inhalative Mn exposure, finally an Mn overflow of the liver is known, resulting in increased Mn transport to other organs, predominantly to the brain. Specific Mn-species then seem to be generated in liver, however, their speciation is still not investigated. This paper focuses on experiments to get more information on Mn species with respect to a size characterization of the Mn species in liver. Liver extracts were analyzed using a mass calibrated size exclusion chromatography (SEC) column being coupled to inductively coupled plasma mass spectrometry (ICP-MS) detection. As an important prerequisite, the stability of Mn species in the liver extracts during storage was investigated as well. It turned out that short term storage of the extract (under Ar atmosphere) at 4 degrees C seems to be best suited. Storage for several days even at -20 degrees C demonstrated already considerable changes in species pattern. Further investigations focused on improvements in detection during hyphenation using dynamic reaction cell (DRC) technology for Mn detection. The signal to noise (S/N) ratio was increased up to a factor of 15 when using DRC technology compared to conventional ICP-MS without DRC. The analysis of liver extracts with a mass calibrated SEC column coupled to inductively coupled plasma-dynamic reaction cell-mass spectrometry (ICP-DRC-MS) showed Mn associated to ca. 36% to a peak covering a mass range 100-260 kDa, approximately 9% was found in a peak having the mass range 37-77 kDa, 46% in a peak having the mass range 13-36 kDa and ca. 7% in the low molecular mass (LMM) range. (c) 2005 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Analytica Chimica Acta	en
dc.identifier.doi	10.1016/j.aca.2005.08.029	en
dc.identifier.isi	ISI:000233496200018	en
dc.identifier.volume	554	en
dc.identifier.issue	1-2	en
dc.identifier.spage	130	en
dc.identifier.epage	135	en