Sporotrichum thermophile type C feruloyl esterase (StFaeC): Purification, characterization, and its use for phenolic acid (sugar) ester synthesis

Topakas, E; Vafiadi, C; Stamatis, H; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Vafiadi, C	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:23:06Z
dc.date.available	2014-03-01T01:23:06Z
dc.date.issued	2005	en
dc.identifier.issn	0141-0229	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16814
dc.subject	Feruloyl esterase	en
dc.subject	Phenolic acid esters	en
dc.subject	Sporotrichum thermophile	en
dc.subject	Surfactantless microemulsions	en
dc.subject	Transesterification	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Alcohols	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Esters	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Ternary systems	en
dc.subject.other	Ferulic acid ester	en
dc.subject.other	Sporotrichum thermophile	en
dc.subject.other	Sporotrichum thermophile type C feruloyl esterase (StFaeC)	en
dc.subject.other	Xylanase	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	4 nitrophenyl 2 o feruloyl alpha levo arabinofuranoside	en
dc.subject.other	4 nitrophenyl 5 o feruloyl alpha levo arabinofuranoside	en
dc.subject.other	arabinofuranose	en
dc.subject.other	arabinose	en
dc.subject.other	butanol	en
dc.subject.other	carbohydrate derivative	en
dc.subject.other	cinnamic acid derivative	en
dc.subject.other	ester	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid	en
dc.subject.other	feruloyl esterase	en
dc.subject.other	fungal enzyme	en
dc.subject.other	hexane	en
dc.subject.other	phenol derivative	en
dc.subject.other	unclassified drug	en
dc.subject.other	water	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	acidity	en
dc.subject.other	alkalinity	en
dc.subject.other	article	en
dc.subject.other	catalysis	en
dc.subject.other	controlled study	en
dc.subject.other	correlation analysis	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme stability	en
dc.subject.other	enzyme subunit	en
dc.subject.other	hydrolysis	en
dc.subject.other	incubation time	en
dc.subject.other	linkage analysis	en
dc.subject.other	nonhuman	en
dc.subject.other	reaction time	en
dc.subject.other	Sporothrix	en
dc.subject.other	sporotrichum thermophile	en
dc.subject.other	synthesis	en
dc.subject.other	wheat bran	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Sporotrichum	en
dc.subject.other	Triticum aestivum	en
dc.title	Sporotrichum thermophile type C feruloyl esterase (StFaeC): Purification, characterization, and its use for phenolic acid (sugar) ester synthesis	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.enzmictec.2004.12.020	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.enzmictec.2004.12.020	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	A feruloyl esterase (StFaeC) produced by Sporotrichum thermophile was purified to homogeneity. The native StFaeC was homodimer with a subunit of M-r 23,000 and pI 3. 1. The enzyme activity was optimal at pH 6.0 and 55 degrees C. The esterase displayed remarkable stability at pH 10.0 and retained 50% of its activity after 133 and 55 min at 55 and 60 degrees C, respectively. Determination of k(cat)/K-m revealed that the enzyme had a broad spectrum of activity against the (hydroxyl) cinnamate esters indicating a type C feruloyl esterase. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose and hydrolysed 4-nitrophenyl-5-O-trans-fer-uloyl-alpha-L-arabinofuranoside three times more efficiently than 4-nitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside. Ferulic acid was efficiently released from wheat bran when the esterase was incubated together with xylanase from S. thermophile (a maximum of 41% total ferulic acid released after 1 h incubation). StFacC by itself could release FA but at a level almost 10-fold lower than that obtained in the presence of xylanase. The potential of StFaeC for the synthesis of various phenolic acid esters was examined using as a reaction system a ternary water-organic mixture consisting of n-hexane, 1-butanol and water. Also StFaeC catalyzed the transfer of the feruloyl group to L-arabinose in a similar system using t-butanol, with about a 40% conversion of L-arabinose to feruloylated derivative was achieved. This work is the first example of enzymatic feruloylation of a carbohydrate. (c) 2004 Elsevier Inc. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	Enzyme and Microbial Technology	en
dc.identifier.doi	10.1016/j.enzmictec.2004.12.020	en
dc.identifier.isi	ISI:000227781000017	en
dc.identifier.volume	36	en
dc.identifier.issue	5-6	en
dc.identifier.spage	729	en
dc.identifier.epage	736	en