Synchrotron micro-FT-IR spectroscopic evaluation of normal paediatric human bone

Petra, M; Anastassopoulou, J; Theologis, T; Theophanides, T

dc.contributor.author	Petra, M	en
dc.contributor.author	Anastassopoulou, J	en
dc.contributor.author	Theologis, T	en
dc.contributor.author	Theophanides, T	en
dc.date.accessioned	2014-03-01T01:23:09Z
dc.date.available	2014-03-01T01:23:09Z
dc.date.issued	2005	en
dc.identifier.issn	0022-2860	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16840
dc.subject	Cortical bone	en
dc.subject	Hydroxyapatite	en
dc.subject	Infrared microspectroscopy	en
dc.subject	Osteons	en
dc.subject	Paediatric	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	Apatites	en
dc.subject.other	Osteons	en
dc.subject.other	Undecalcified cortical bones	en
dc.subject.other	Biopsy	en
dc.subject.other	Carbonates	en
dc.subject.other	Fourier transform infrared spectroscopy	en
dc.subject.other	Molecular structure	en
dc.subject.other	Phosphates	en
dc.subject.other	Bone	en
dc.subject.other	amide	en
dc.subject.other	apatite	en
dc.subject.other	carbonic acid	en
dc.subject.other	phosphate	en
dc.subject.other	article	en
dc.subject.other	bone biopsy	en
dc.subject.other	bone tissue	en
dc.subject.other	calculation	en
dc.subject.other	femur trochanteric fracture	en
dc.subject.other	Haversian canal	en
dc.subject.other	human	en
dc.subject.other	human tissue	en
dc.subject.other	infrared spectroscopy	en
dc.subject.other	intraoperative period	en
dc.subject.other	male	en
dc.subject.other	pediatrics	en
dc.subject.other	precipitation	en
dc.subject.other	school child	en
dc.subject.other	synchrotron	en
dc.title	Synchrotron micro-FT-IR spectroscopic evaluation of normal paediatric human bone	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.molstruc.2004.07.041	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.molstruc.2004.07.041	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	Cortical and cancellous normal bone biopsies were obtained intra-operatively from the inter-trochanteric region of an 11-year old boy and were studied with FT-IR spectroscopy. Undecalcified cortical bone sections, in which the micro-structure of the tissue had been preserved, were studied with synchrotron micro-FT-IR spectroscopy. The Amide I, II and III bands showed peaks near 1650, 1550 and 1240 cm(-1) respectively. The v(1), v(2) and v(3) phosphate and carbonate groups absorbed in the regions 1200-900 and 900-850 cm(-1), respectively. Calculation of the ratio of the integrated areas under the v(1), v(3) phosphate and Amide I peaks, as a function of changing distance from the centre of the osteon, was used as an index of the relative mineral to matrix presence at each site of micro-FT-IR spectroscopic analysis. The ratio of the integrated areas under the v(2) carbonate and v(1), v(3) phosphate peaks, as a function of changing distance from the centre of the osteon was used as an index of the relative carbonate to phosphate groups presence at each site of analysis. Both ratios were found to consistently increase with increasing distance from the centre of the osteon, for all osteons examined. In some fresh osteons, a peak near 1125 cm(-1), characteristic of newly precipitated apatites (PO43-) was identified for the first time in the FT-IR spectra obtained from the inner lamellae of each Haversian system. This may be attributed to crystal effects of in vivo environments of fresh paediatric osteons. (C) 2004 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Molecular Structure	en
dc.identifier.doi	10.1016/j.molstruc.2004.07.041	en
dc.identifier.isi	ISI:000225487000015	en
dc.identifier.volume	733	en
dc.identifier.issue	1-3	en
dc.identifier.spage	101	en
dc.identifier.epage	110	en