The influence of different cultivation conditions on the metabolome of Fusarium oxysporum

Panagiotou, G; Christakopoulos, P; Olsson, L

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Olsson, L	en
dc.date.accessioned	2014-03-01T01:23:11Z
dc.date.available	2014-03-01T01:23:11Z
dc.date.issued	2005	en
dc.identifier.issn	0168-1656	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/16861
dc.subject	Different carbon sources	en
dc.subject	Fusarium oxysporum	en
dc.subject	Metabolite profile	en
dc.subject	Principal component analysis	en
dc.subject	Redox balance	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Amino acids	en
dc.subject.other	Ethanol	en
dc.subject.other	Redox reactions	en
dc.subject.other	Substrates	en
dc.subject.other	Cultivation conditions	en
dc.subject.other	Ethanol production	en
dc.subject.other	Cultivation	en
dc.subject.other	acetic acid	en
dc.subject.other	alcohol	en
dc.subject.other	ammonia	en
dc.subject.other	arabinose	en
dc.subject.other	glucose 1 phosphate	en
dc.subject.other	nitrate	en
dc.subject.other	nitrogen	en
dc.subject.other	pentose	en
dc.subject.other	pentose phosphate	en
dc.subject.other	reduced nicotinamide adenine dinucleotide	en
dc.subject.other	reduced nicotinamide adenine dinucleotide phosphate	en
dc.subject.other	xylitol	en
dc.subject.other	xylose	en
dc.subject.other	alcohol production	en
dc.subject.other	article	en
dc.subject.other	carbon source	en
dc.subject.other	controlled study	en
dc.subject.other	culture optimization	en
dc.subject.other	denitrification	en
dc.subject.other	fungal cell culture	en
dc.subject.other	fungal metabolism	en
dc.subject.other	fungal strain	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	metabolite	en
dc.subject.other	metabolomics	en
dc.subject.other	nonhuman	en
dc.subject.other	oxidation reduction reaction	en
dc.subject.other	oxygen supply	en
dc.subject.other	pentose phosphate cycle	en
dc.subject.other	principal component analysis	en
dc.subject.other	priority journal	en
dc.subject.other	Adaptation, Physiological	en
dc.subject.other	Cell Culture Techniques	en
dc.subject.other	Fungal Proteins	en
dc.subject.other	Fusarium	en
dc.subject.other	Gene Expression Profiling	en
dc.subject.other	Gene Expression Regulation, Fungal	en
dc.subject.other	Proteome	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium oxysporum	en
dc.title	The influence of different cultivation conditions on the metabolome of Fusarium oxysporum	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jbiotec.2005.05.004	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.jbiotec.2005.05.004	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	The two most widespread pentose sugars found in the biosphere are D-xylose and L-arabinose. They are both potential substrates for ethanol production. The purpose of this study was to better understand the redox constraints imposed to Fusarium oxysporum during utilization of pentoses. In order to increase ethanol yield and decrease by-product formation, nitrate was used as nitrogen source. The use of NADH, the cofactor in denitrification process when using nitrate as a nitrogen source, improved the ethanol yield on xylose to 0.89 mol mol(-1) compared to the ethanol yield achieved using ammonium as nitrogen source 0.44 mol mol(-1). The improved ethanol yield was followed by a 28% decrease in yield of the by-product xylitol. In order to investigate the metabolic pathway of arabinose and the metabolic limitations for the efficient ethanol production from this sugar, the extracellular and intracellular metabolite profiles were determined under aerobic and anaerobic cultivation conditions. The results of this study clearly show difficulties in channelling of glucose-1-P (G1P) to pentose phosphate pathway (PPP) and reduced NADPH regeneration, suggesting that NADPH becomes a limiting factor for arabinose conversion, resulting in excessive acetate production. Variations of the fungus intracellular amino and non-amino acid pool, under different culture conditions, were evaluated using principal component analysis (PCA). PCA projection of the metabolome data collected from F. oxysporum subjected to environmental perturbations succeeded to visualize different physiological states and the conclusions of this study were that the metabolite profile is unique according to: (1) the carbon source and (2) the oxygen supply, and to a lesser extent to the cultivation phase. (C) 2005 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Biotechnology	en
dc.identifier.doi	10.1016/j.jbiotec.2005.05.004	en
dc.identifier.isi	ISI:000231124200008	en
dc.identifier.volume	118	en
dc.identifier.issue	3	en
dc.identifier.spage	304	en
dc.identifier.epage	315	en