Analysis of size characterized manganese species from liver extracts using capillary zone electrophoresis coupled to inductively coupled plasma mass spectrometry (CZE-ICP-MS)

Quintana, M; Klouda, AD; Gondikas, A; Ochsenkuhn-Petropoulou, M; Michalke, B

dc.contributor.author	Quintana, M	en
dc.contributor.author	Klouda, AD	en
dc.contributor.author	Gondikas, A	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.contributor.author	Michalke, B	en
dc.date.accessioned	2014-03-01T01:23:36Z
dc.date.available	2014-03-01T01:23:36Z
dc.date.issued	2006	en
dc.identifier.issn	0003-2670	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17042
dc.subject	Capillary zone electrophoresis	en
dc.subject	Inductively coupled plasma mass spectrometry	en
dc.subject	Liver extract	en
dc.subject	Manganese	en
dc.subject	Speciation	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Biological organs	en
dc.subject.other	Electrophoresis	en
dc.subject.other	Extraction	en
dc.subject.other	Inductively coupled plasma	en
dc.subject.other	Mass spectrometry	en
dc.subject.other	Physiology	en
dc.subject.other	Size exclusion chromatography	en
dc.subject.other	Toxic materials	en
dc.subject.other	Capillary zone electrophoresis	en
dc.subject.other	Inductively coupled plasma mass spectrometry	en
dc.subject.other	Liver extract	en
dc.subject.other	Speciation	en
dc.subject.other	Manganese	en
dc.subject.other	arginase	en
dc.subject.other	galactosyltransferase	en
dc.subject.other	isocitrate dehydrogenase	en
dc.subject.other	liver extract	en
dc.subject.other	manganese	en
dc.subject.other	proline dipeptidase	en
dc.subject.other	pyruvate carboxylase	en
dc.subject.other	animal tissue	en
dc.subject.other	article	en
dc.subject.other	capillary zone electrophoresis	en
dc.subject.other	chemical analysis	en
dc.subject.other	chromatography	en
dc.subject.other	controlled study	en
dc.subject.other	elution	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	liver level	en
dc.subject.other	mass spectrometry	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	suction	en
dc.title	Analysis of size characterized manganese species from liver extracts using capillary zone electrophoresis coupled to inductively coupled plasma mass spectrometry (CZE-ICP-MS)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.aca.2006.02.045	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.aca.2006.02.045	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	Mn is of toxicological concern because overexposure can lead to progressive, permanent neurodegenerative damage. Monomethyl-Mnpentadienyl-tricarbonyl (MMT) is used as an anti-knock agent in fuel. Exhausted Mn compounds are absorbed in the lung and transported to the liver. Extended exposure causes an overflow of the liver with Mn species moving e.g. to the brain, causing irreversible central nervous system (CNS) disorders like Manganism. This paper focuses on experiments for getting more information on Mn species in liver extracts. The investigations are performed with respect to (1) a size characterization and (2) a subsequent identification of the Mn species in liver extracts using preparative size exclusion chromatography (SEC) followed by capillary zone electrophoresis coupled to inductively coupled plasma mass spectrometry (CZE-ICP-MS). First, extracts were analyzed using a mass calibrated SEC column coupled to ICP-MS detection. The chromatograrn showed the Mn-55-trace and proved main Mn elution between ca. 60-150 kDa. Second, liver extracts were fractionated on the same SEC column, however, now the effluent was directed to a fraction collector. This resulted in fractions containing pre-purified, size characterized Mn species per fraction. It turned out that the Mn concentrations per fraction reflected roughly the previous on-line Mn trace. Third, the fractions were subject to CZE-ICP-MS, where the MS was operated additionally with dynamic reaction cell (DRC) technique. From size characterization (with SEC coupled on-line to ICP-MS or connected to a fraction collector and subsequent Mn determination in fractions) it was shown that most Mn species from liver extract were of high molecular mass (HMM) nature as they eluted mostly between 50 and 80 min, corresponding to ca. 60-150 kDa. With the two-dimensional speciation approach employing first SEC and then CZE-ICP-DRC-MS together with standard addition method, a series of Mn species was identified. Mn species predominantly were Mn-enzymes e.g. arginase, isocitric dehydrogenase, galactosyltransferase, prolidase, pyruvate carboxylase and oxalate oxidase. A typical Mn-transporter -Mn-alburnin-was also seen, whilst Mn-transferrin obviously was degraded during SEC separation. This Mn-compound (independent whether as a standard or from liver extract) was not stable during SEC even at the finally chosen physiological conditions. (c) 2006 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Analytica Chimica Acta	en
dc.identifier.doi	10.1016/j.aca.2006.02.045	en
dc.identifier.isi	ISI:000239524800026	en
dc.identifier.volume	573-574	en
dc.identifier.spage	172	en
dc.identifier.epage	180	en