Encapsulation of biomolecules for bioanalytical purposes: Preparation of diclofenac antibody-doped nanometer-sized silica particles by reverse micelle and sol-gel processing

Tsagkogeorgas, F; Ochsenkuhn-Petropoulou, M; Niessner, R; Knopp, D

dc.contributor.author	Tsagkogeorgas, F	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.contributor.author	Niessner, R	en
dc.contributor.author	Knopp, D	en
dc.date.accessioned	2014-03-01T01:24:18Z
dc.date.available	2014-03-01T01:24:18Z
dc.date.issued	2006	en
dc.identifier.issn	0003-2670	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17204
dc.subject	Antibodies	en
dc.subject	Diclofenac	en
dc.subject	Encapsulation	en
dc.subject	Reverse micelle	en
dc.subject	Silica nanoparticles	en
dc.subject	Sol-gel	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Biochemistry	en
dc.subject.other	Doping (additives)	en
dc.subject.other	Emulsions	en
dc.subject.other	Micelles	en
dc.subject.other	Nanostructured materials	en
dc.subject.other	Silica	en
dc.subject.other	Sol-gels	en
dc.subject.other	Diclofenac	en
dc.subject.other	Reverse micelle	en
dc.subject.other	Silica nanoparticles	en
dc.subject.other	Antibodies	en
dc.subject.other	cyclohexane	en
dc.subject.other	diclofenac	en
dc.subject.other	diclofenac antibody	en
dc.subject.other	drug antibody	en
dc.subject.other	immobilized antibody	en
dc.subject.other	immunoglobulin G	en
dc.subject.other	nanoparticle	en
dc.subject.other	nonionic surfactant	en
dc.subject.other	organic solvent	en
dc.subject.other	polyclonal antiserum	en
dc.subject.other	polyoxyethylene(5)nonylphenylether	en
dc.subject.other	rabbit antiserum	en
dc.subject.other	silicon dioxide	en
dc.subject.other	tetramethoxysilane	en
dc.subject.other	unclassified drug	en
dc.subject.other	analytic method	en
dc.subject.other	article	en
dc.subject.other	biological activity	en
dc.subject.other	encapsulation	en
dc.subject.other	enzyme linked immunosorbent assay	en
dc.subject.other	filtration	en
dc.subject.other	microemulsion	en
dc.subject.other	particle size	en
dc.subject.other	precursor	en
dc.subject.other	priority journal	en
dc.subject.other	processing	en
dc.subject.other	reverse micelle	en
dc.subject.other	scanning electron microscopy	en
dc.subject.other	sol gel processing	en
dc.subject.other	transmission electron microscopy	en
dc.subject.other	X ray fluorescence	en
dc.subject.other	Oryctolagus cuniculus	en
dc.title	Encapsulation of biomolecules for bioanalytical purposes: Preparation of diclofenac antibody-doped nanometer-sized silica particles by reverse micelle and sol-gel processing	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.aca.2006.03.006	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.aca.2006.03.006	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	In recent years, the sol-gel technique has attracted increasing interest as a unique approach to immobilize biomolecules for bioanalytical applications as well as biochemical and biophysical studies. For this purpose, crushed biomolecule-doped sol-gel glass monoliths have been widely used. In the present work, for the first time, the encapsulation of anti-diclofenac antibodies in silica nanoparticles was carried out by a combination of reverse micelle and sol-gel technique. Cyclohexane was used for the preparation of the microemulsion as organic solvent, while surfactant Igepal CO-520 was found to be the optimal stabilizer. The antibody source was a purified IgG fraction originating from a polyclonal rabbit antiserum. Tetramethyl orthosilicate (TMOS) was used as precursor. Rather uniform, monodispersed and spherical silica particles of about 70 nm diameter size were fabricated, as was demonstrated by transmission electron microscopy (TEM) and scanning electron microscopy/energy dispersive X-ray fluorescence analysis (SEM/EDX). The biological activity of the encapsulated antibodies was evaluated by incubation of the nanoparticles with a diclofenac standard solution and analysis of the filtrate and followed washing solutions by a highly sensitive enzyme-linked immunosorbent assay (ELISA), using non-doped particles as blanks. While only about 6% of the added diclofenac was nonspecifically retained by the blank, the corresponding amount of about 66% was much higher with the antibody-doped particles. An obvious advantage of this approach is the general applicability of the developed technique for a mild immobilization of different antibody species. (c) 2006 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Analytica Chimica Acta	en
dc.identifier.doi	10.1016/j.aca.2006.03.006	en
dc.identifier.isi	ISI:000239524800021	en
dc.identifier.volume	573-574	en
dc.identifier.spage	133	en
dc.identifier.epage	137	en