Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose

Panagiotou, G; Christakopoulos, P; Grotkjaer, T; Olsson, L

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Grotkjaer, T	en
dc.contributor.author	Olsson, L	en
dc.date.accessioned	2014-03-01T01:24:19Z
dc.date.available	2014-03-01T01:24:19Z
dc.date.issued	2006	en
dc.identifier.issn	1096-7176	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17210
dc.subject	13C-labelling experiments	en
dc.subject	Fusarium oxysporum	en
dc.subject	Metabolic network analysis	en
dc.subject	Nitrogen source	en
dc.subject	Redox balance	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Ethanol	en
dc.subject.other	Metabolism	en
dc.subject.other	Nitrates	en
dc.subject.other	Reduction	en
dc.subject.other	Xylose	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Metabolic network analysis	en
dc.subject.other	Nitrogen source	en
dc.subject.other	Redox balance	en
dc.subject.other	Fungi	en
dc.subject.other	carbon	en
dc.subject.other	nicotinamide adenine dinucleotide	en
dc.subject.other	nitrogen	en
dc.subject.other	reduced nicotinamide adenine dinucleotide phosphate	en
dc.subject.other	xylitol	en
dc.subject.other	xylose	en
dc.subject.other	anaerobic capacity	en
dc.subject.other	anaerobic cell culture	en
dc.subject.other	article	en
dc.subject.other	batch cell culture	en
dc.subject.other	carbon metabolism	en
dc.subject.other	chemostat	en
dc.subject.other	citric acid cycle	en
dc.subject.other	controlled study	en
dc.subject.other	denitrification	en
dc.subject.other	fungus culture	en
dc.subject.other	fungus growth	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	genetic engineering	en
dc.subject.other	metabolic engineering	en
dc.subject.other	microbial biomass	en
dc.subject.other	nonhuman	en
dc.subject.other	oxidation reduction reaction	en
dc.subject.other	priority journal	en
dc.subject.other	steady state	en
dc.subject.other	Anaerobiosis	en
dc.subject.other	Bioreactors	en
dc.subject.other	Cell Culture Techniques	en
dc.subject.other	Cell Proliferation	en
dc.subject.other	Fusarium	en
dc.subject.other	Genetic Enhancement	en
dc.subject.other	Nitrates	en
dc.subject.other	Oxidation-Reduction	en
dc.subject.other	Xylose	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium oxysporum	en
dc.title	Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ymben.2006.04.004	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.ymben.2006.04.004	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	Dissimilatory nitrate reduction metabolism, of the natural xylose-fermenting fungus Fusarium oxysporum, was used as a strategy to achieve anaerobic growth and ethanol production from xylose. Beneficial alterations of the redox fluxes and thereby of the xylose metabolism were obtained by taking advantage of the regeneration of the cofactor NAD(+) during the denitrification process. In batch cultivations, nitrate sustained growth under anaerobic conditions (1.21 g L-1 biomass) and simultaneously a maximum yield of 0.55 moles of ethanol per mole of xylose was achieved, whereas substitution of nitrate with ammonium limited the growth significantly (0.15 g L-1 biomass). Using nitrate, the maximum acetate yield was 0.21 moles per mole of xylose and no xylitol excretion was observed. Furthermore, the network structure in the central carbon metabolism of F. oxysporum was characterized in steady state. F. oxysporum grew anaerobically on [1-C-13] labelled glucose and unlabelled xylose in chemostat cultivation with nitrate as nitrogen source. The use of labelled substrate allowed the precise determination of the glucose and xylose contribution to the carbon fluxes in the central metabolism of this poorly described microorganism. It was demonstrated that dissimilatory nitrate reduction allows F oxysporum to exhibit typical respiratory metabolic behaviour with a highly active TCA cycle and a large demand for NADPH. (C) 2006 Elsevier Inc. All rights reserved.	en
heal.publisher	ACADEMIC PRESS INC ELSEVIER SCIENCE	en
heal.journalName	Metabolic Engineering	en
dc.identifier.doi	10.1016/j.ymben.2006.04.004	en
dc.identifier.isi	ISI:000240600800009	en
dc.identifier.volume	8	en
dc.identifier.issue	5	en
dc.identifier.spage	474	en
dc.identifier.epage	482	en