Evaluation of trapping efficiency of optical tweezers by dielectrophoresis

Papagiakoumou, E; Pietreanu, D; Makropoulou, MI; Kovacs, E; Serafetinides, AA

dc.contributor.author	Papagiakoumou, E	en
dc.contributor.author	Pietreanu, D	en
dc.contributor.author	Makropoulou, MI	en
dc.contributor.author	Kovacs, E	en
dc.contributor.author	Serafetinides, AA	en
dc.date.accessioned	2014-03-01T01:24:22Z
dc.date.available	2014-03-01T01:24:22Z
dc.date.issued	2006	en
dc.identifier.issn	1083-3668	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17231
dc.subject	Dielectrophoresis	en
dc.subject	Force calibration	en
dc.subject	Optical tweezers	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Optics	en
dc.subject.classification	Radiology, Nuclear Medicine & Medical Imaging	en
dc.subject.other	Brownian movement	en
dc.subject.other	Calibration	en
dc.subject.other	Drag	en
dc.subject.other	Electrophoresis	en
dc.subject.other	Microelectrodes	en
dc.subject.other	Photolithography	en
dc.subject.other	Polystyrenes	en
dc.subject.other	Dielectrophoresis	en
dc.subject.other	Force calibration	en
dc.subject.other	Optical tweezers	en
dc.subject.other	Optical instruments	en
dc.title	Evaluation of trapping efficiency of optical tweezers by dielectrophoresis	en
heal.type	journalArticle	en
heal.identifier.primary	10.1117/1.2165176	en
heal.identifier.secondary	http://dx.doi.org/10.1117/1.2165176	en
heal.identifier.secondary	014035	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	A relatively new method for measuring optically induced forces on microparticles and cells, different from the conventional Brownian motion and viscous drag force calibration methods widely used, is introduced. It makes use of the phenomenon of dielectro-phoresis for the calibration of optical tweezers through the dielectrophoretic force calculations. A pair of microelectrodes is fabricated by photolithography on a microscope slide and it is connected to a high-frequency generator. The calibration of the optical tweezers setup is performed by the manipulation of polystyrene beads and yeast cells. Calibration diagrams of the transverse forces versus power are deduced for different cell radii and numerical apertures of the objective lenses. The optical system and the related technique provide a fast and easy method for optical tweezers calibration. (c) 2006 Society of PhotoOptical Instrumentation Engineers.	en
heal.publisher	SPIE-INT SOCIETY OPTICAL ENGINEERING	en
heal.journalName	Journal of Biomedical Optics	en
dc.identifier.doi	10.1117/1.2165176	en
dc.identifier.isi	ISI:000236173100036	en
dc.identifier.volume	11	en
dc.identifier.issue	1	en