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Evaluation of trapping efficiency of optical tweezers by dielectrophoresis

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dc.contributor.author Papagiakoumou, E en
dc.contributor.author Pietreanu, D en
dc.contributor.author Makropoulou, MI en
dc.contributor.author Kovacs, E en
dc.contributor.author Serafetinides, AA en
dc.date.accessioned 2014-03-01T01:24:22Z
dc.date.available 2014-03-01T01:24:22Z
dc.date.issued 2006 en
dc.identifier.issn 1083-3668 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/17231
dc.subject Dielectrophoresis en
dc.subject Force calibration en
dc.subject Optical tweezers en
dc.subject.classification Biochemical Research Methods en
dc.subject.classification Optics en
dc.subject.classification Radiology, Nuclear Medicine & Medical Imaging en
dc.subject.other Brownian movement en
dc.subject.other Calibration en
dc.subject.other Drag en
dc.subject.other Electrophoresis en
dc.subject.other Microelectrodes en
dc.subject.other Photolithography en
dc.subject.other Polystyrenes en
dc.subject.other Dielectrophoresis en
dc.subject.other Force calibration en
dc.subject.other Optical tweezers en
dc.subject.other Optical instruments en
dc.title Evaluation of trapping efficiency of optical tweezers by dielectrophoresis en
heal.type journalArticle en
heal.identifier.primary 10.1117/1.2165176 en
heal.identifier.secondary http://dx.doi.org/10.1117/1.2165176 en
heal.identifier.secondary 014035 en
heal.language English en
heal.publicationDate 2006 en
heal.abstract A relatively new method for measuring optically induced forces on microparticles and cells, different from the conventional Brownian motion and viscous drag force calibration methods widely used, is introduced. It makes use of the phenomenon of dielectro-phoresis for the calibration of optical tweezers through the dielectrophoretic force calculations. A pair of microelectrodes is fabricated by photolithography on a microscope slide and it is connected to a high-frequency generator. The calibration of the optical tweezers setup is performed by the manipulation of polystyrene beads and yeast cells. Calibration diagrams of the transverse forces versus power are deduced for different cell radii and numerical apertures of the objective lenses. The optical system and the related technique provide a fast and easy method for optical tweezers calibration. (c) 2006 Society of PhotoOptical Instrumentation Engineers. en
heal.publisher SPIE-INT SOCIETY OPTICAL ENGINEERING en
heal.journalName Journal of Biomedical Optics en
dc.identifier.doi 10.1117/1.2165176 en
dc.identifier.isi ISI:000236173100036 en
dc.identifier.volume 11 en
dc.identifier.issue 1 en


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