Investigation of red natural dyes used in historical objects by HPLC-DAD-MS

Karapanagiotis, I; Chryssoulakis, Y

dc.contributor.author	Karapanagiotis, I	en
dc.contributor.author	Chryssoulakis, Y	en
dc.date.accessioned	2014-03-01T01:24:32Z
dc.date.available	2014-03-01T01:24:32Z
dc.date.issued	2006	en
dc.identifier.issn	0003-4592	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17315
dc.subject.classification	Chemistry, Analytical	en
dc.subject.classification	Environmental Sciences	en
dc.subject.other	alizarin	en
dc.subject.other	anthraquinone derivative	en
dc.subject.other	carminic acid	en
dc.subject.other	coloring agent	en
dc.subject.other	drug derivative	en
dc.subject.other	pigment	en
dc.subject.other	purpurin	en
dc.subject.other	archeology	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	electrospray mass spectrometry	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	history	en
dc.subject.other	methodology	en
dc.subject.other	Southern Europe	en
dc.subject.other	ultraviolet spectrophotometry	en
dc.subject.other	Anthraquinones	en
dc.subject.other	Archaeology	en
dc.subject.other	Carmine	en
dc.subject.other	Chromatography, High Pressure Liquid	en
dc.subject.other	Coloring Agents	en
dc.subject.other	History, Ancient	en
dc.subject.other	Mediterranean Region	en
dc.subject.other	Pigments, Biological	en
dc.subject.other	Spectrometry, Mass, Electrospray Ionization	en
dc.subject.other	Spectrophotometry, Ultraviolet	en
dc.subject.other	Caesalpinia echinata	en
dc.subject.other	Carthamus tinctorius	en
dc.subject.other	Rubia tinctorum	en
dc.title	Investigation of red natural dyes used in historical objects by HPLC-DAD-MS	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/adic.200690008	en
heal.identifier.secondary	http://dx.doi.org/10.1002/adic.200690008	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	High performance liquid chromatography (HPLC) with UV-Vis Diode Array Detection (DAD) and electrospray mass spectrometric (ESI-MS) method was utilized for the identification of coloring components of madder, Armenian and Mexican cochineal, lac dye, brazilwood, safflower and dragon blood - probably the most important red natural dyestuffs found in objects of the cultural heritage. UV-Vis detection limits in the range of 0.2-0.6 ng for carminic acid, alizarin and purpurin were achieved using a gradient elution of H2O-0.01%TFA and CH3CN-0.01%TFA. ESI mass spectrometer was also used, as a supportive detection method to the standard DAD, for further analysis of the tested materials, with the ability to analyze dyestuffs as small as one milligram. The presence of madder was revealed in two historical (Hellenistic and Roman period) samples, found in the Mediterranean area, by identifying purpurin in both of them. Munjistin was also identified in one of the samples (Hellenistic period) while alizarin was not detected, raising questions regarding the exact madder type, utilized in the historical samples.	en
heal.publisher	SOC CHIMICA ITALIANA	en
heal.journalName	Annali di Chimica	en
dc.identifier.doi	10.1002/adic.200690008	en
dc.identifier.isi	ISI:000237015400006	en
dc.identifier.volume	96	en
dc.identifier.issue	1-2	en
dc.identifier.spage	75	en
dc.identifier.epage	84	en