Kenaf xylan - A source of biologically active acidic oligosaccharides

Nacos, MK; Katapodis, P; Pappas, C; Daferera, D; Tarantilis, PA; Christakopoulos, P; Polissiou, M

dc.contributor.author	Nacos, MK	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Pappas, C	en
dc.contributor.author	Daferera, D	en
dc.contributor.author	Tarantilis, PA	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Polissiou, M	en
dc.date.accessioned	2014-03-01T01:24:32Z
dc.date.available	2014-03-01T01:24:32Z
dc.date.issued	2006	en
dc.identifier.issn	0144-8617	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17321
dc.subject	13C NMR spectroscopy	en
dc.subject	Endoxylanase	en
dc.subject	Extraction	en
dc.subject	FT-IR spectroscopy	en
dc.subject	Hemicellulose	en
dc.subject	Kenaf	en
dc.subject	Oligosaccharide	en
dc.subject	Uronic acid	en
dc.subject	Xylan	en
dc.subject.classification	Chemistry, Applied	en
dc.subject.classification	Chemistry, Organic	en
dc.subject.classification	Polymer Science	en
dc.subject.other	Cellulose	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Fourier transform infrared spectroscopy	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Nuclear magnetic resonance spectroscopy	en
dc.subject.other	Aldouronic acids	en
dc.subject.other	Endoxylanase	en
dc.subject.other	Hemicellulose	en
dc.subject.other	Oligomers	en
dc.subject.other	Infrared Spectra	en
dc.subject.other	Kenaf	en
dc.subject.other	Oligosaccharides	en
dc.subject.other	Uronic Acids	en
dc.subject.other	Xylans	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Hibiscus	en
dc.subject.other	Hibiscus cannabinus	en
dc.subject.other	Sporothrix	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Kenaf xylan - A source of biologically active acidic oligosaccharides	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.carbpol.2006.02.032	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.carbpol.2006.02.032	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	Two xylose-rich hemicellulose fractions were obtained from kenaf wood (Hibiscus cannabinus L.) through a series of sequential extractions which dissociated xylans from other cell wall components. These fractions were subsequently used as substrates for the production of biologically active aldouronic acids. Incubation of the xylans with a family 10 Thermoascus aurantiacus endoxylanase resulted in the isolation of an aldotetrauronic acid as the main acidic oligosaccharide in the hydrolysis products. Enzymic hydrolysis with a family 11 Sporotrichum thermophile endoxylanase instead resulted in the isolation of a aldopentauronic acid as the main acidic oligosaccharide. The identity and purity of both xylans and aldouronic acids were assessed with solid-state FT-IR, solid-state and solution C-13 NMR spectroscopy. (c) 2006 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Carbohydrate Polymers	en
dc.identifier.doi	10.1016/j.carbpol.2006.02.032	en
dc.identifier.isi	ISI:000241444900017	en
dc.identifier.volume	66	en
dc.identifier.issue	1	en
dc.identifier.spage	126	en
dc.identifier.epage	134	en