Mastic oil from Pistacia lentiscus var. chia inhibits growth and survival of human K562 leukemia cells and attenuates angiogenesis

Loutrari, H; Magkouta, S; Pyriochou, A; Koika, V; Kolisis, FN; Papapetropoulos, A; Roussos, C

dc.contributor.author	Loutrari, H	en
dc.contributor.author	Magkouta, S	en
dc.contributor.author	Pyriochou, A	en
dc.contributor.author	Koika, V	en
dc.contributor.author	Kolisis, FN	en
dc.contributor.author	Papapetropoulos, A	en
dc.contributor.author	Roussos, C	en
dc.date.accessioned	2014-03-01T01:24:35Z
dc.date.available	2014-03-01T01:24:35Z
dc.date.issued	2006	en
dc.identifier.issn	0163-5581	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17349
dc.subject.classification	Oncology	en
dc.subject.classification	Nutrition & Dietetics	en
dc.subject.other	essential oil	en
dc.subject.other	food additive	en
dc.subject.other	mastic oil	en
dc.subject.other	mitogen activated protein kinase 1	en
dc.subject.other	mitogen activated protein kinase 3	en
dc.subject.other	perillyl alcohol	en
dc.subject.other	pistacia lentiscus extract	en
dc.subject.other	plant extract	en
dc.subject.other	RhoA guanine nucleotide binding protein	en
dc.subject.other	unclassified drug	en
dc.subject.other	vasculotropin	en
dc.subject.other	angiogenesis	en
dc.subject.other	animal cell	en
dc.subject.other	antineoplastic activity	en
dc.subject.other	apoptosis	en
dc.subject.other	article	en
dc.subject.other	cancer inhibition	en
dc.subject.other	cancer prevention	en
dc.subject.other	cell proliferation	en
dc.subject.other	cell strain K 562	en
dc.subject.other	cell survival	en
dc.subject.other	concentration response	en
dc.subject.other	controlled study	en
dc.subject.other	diet supplementation	en
dc.subject.other	drug mechanism	en
dc.subject.other	endothelium cell	en
dc.subject.other	human	en
dc.subject.other	human cell	en
dc.subject.other	in vitro study	en
dc.subject.other	in vivo study	en
dc.subject.other	leukemia cell	en
dc.subject.other	melanoma B16	en
dc.subject.other	mouse	en
dc.subject.other	nonhuman	en
dc.subject.other	Pistacia lentiscus	en
dc.subject.other	protein secretion	en
dc.subject.other	time	en
dc.subject.other	Angiogenesis Inhibitors	en
dc.subject.other	Animals	en
dc.subject.other	Antineoplastic Agents	en
dc.subject.other	Apoptosis	en
dc.subject.other	Blotting, Western	en
dc.subject.other	Cell Division	en
dc.subject.other	Cell Line, Tumor	en
dc.subject.other	Dose-Response Relationship, Drug	en
dc.subject.other	Enzyme-Linked Immunosorbent Assay	en
dc.subject.other	Humans	en
dc.subject.other	K562 Cells	en
dc.subject.other	Melanoma, Experimental	en
dc.subject.other	Mice	en
dc.subject.other	Monoterpenes	en
dc.subject.other	Pistacia	en
dc.subject.other	Plant Oils	en
dc.subject.other	Time Factors	en
dc.subject.other	Vascular Endothelial Growth Factor A	en
dc.subject.other	Pistacia lentiscus	en
dc.title	Mastic oil from Pistacia lentiscus var. chia inhibits growth and survival of human K562 leukemia cells and attenuates angiogenesis	en
heal.type	journalArticle	en
heal.identifier.primary	10.1207/s15327914nc5501_11	en
heal.identifier.secondary	http://dx.doi.org/10.1207/s15327914nc5501_11	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	Mastic oil from Pistacia lentiscus var. chia, a natural plant extract traditionally used as a food additive, has been extensively studied for its antimicrobial activity attributed to the combination of its bioactive components. One of them, perillyl alcohol (POH), displays tumor chemopreventive, chemotherapeutic, and antiangiogenic properties. We investigated whether mastic oil would also suppress tumor cell growth and angiogenesis. We observed that mastic oil concentration and time dependently exerted an antiproliferative and proapoptotic effect on K562 human leukemia cells and inhibited the release of vascular endothelial growth factor (VEGF) from K562 and B16 mouse melanoma cells. Moreover, mastic oil caused a concentration-dependent inhibition of endothelial cell (EC) proliferation without affecting cell survival and a significant decrease of microvessel formation both in vitro and in vivo. Investigation of underlying mechanism(s) demonstrated that mastic oil reduced 1) in K562 cells the activation of extracellular signal-regulated kinases 1/2 (Erk1/2) known to control leukemia cell proliferation, survival, and VEGF secretion and 2) in EC the activation of RhoA, an essential regulator of neovessel organization. Overall, our results underscore that mastic oil, through its multiple effects on malignant cells and ECs, may be a useful natural dietary supplement for cancer prevention. Copyright © 2006, Lawrence Erlbaum Associates, Inc.	en
heal.publisher	LAWRENCE ERLBAUM ASSOC INC	en
heal.journalName	Nutrition and Cancer	en
dc.identifier.doi	10.1207/s15327914nc5501_11	en
dc.identifier.isi	ISI:000240662000011	en
dc.identifier.volume	55	en
dc.identifier.issue	1	en
dc.identifier.spage	86	en
dc.identifier.epage	93	en