Optimization of xylanase production by Thermomyces lanuginosus in tomato seed meal using response surface methodology

Katapodis, P; Christakopoulou, V; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Christakopoulou, V	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:24:49Z
dc.date.available	2014-03-01T01:24:49Z
dc.date.issued	2006	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17450
dc.subject	Response surface method	en
dc.subject	Thermomyces lanuginosus	en
dc.subject	Tomato seed meal	en
dc.subject	Waste utilization	en
dc.subject	Xylanase	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Bioreactors	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Meats	en
dc.subject.other	Optimization	en
dc.subject.other	Seed	en
dc.subject.other	Yeast	en
dc.subject.other	Response surface method	en
dc.subject.other	Thermomyces lanuginosus	en
dc.subject.other	Tomato seed meal	en
dc.subject.other	Xylanase	en
dc.subject.other	Crops	en
dc.subject.other	Enzymes	en
dc.subject.other	Tomatoes	en
dc.subject.other	Yeasts	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	Thermomyces lanuginosus	en
dc.subject.other	Zea mays	en
dc.title	Optimization of xylanase production by Thermomyces lanuginosus in tomato seed meal using response surface methodology	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11274-005-9063-7	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s11274-005-9063-7	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	A 32 central composite experimental design was performed with the aim of optimizing xylanase production by Thermomyces lanuginosus grown on corn cobs in submerged cultures. Xylanase production was first tested on different nitrogen sources (tomato skin, tomato seed meal, corn steep liquor, meat peptone, bacto-tryptone and yeast extract). Tomato seed meal was the selected substrate to test the effect of two variables on xylanase production (corn cobs and tomato seed meal concentrations). A second-order quadratic model and a response surface method showed that the optimum condition for xylanase production was corn cobs 4.6% (w/v) and tomato seed meal 2.1% (w/v). The optimum conditions found were transferred to 7-1 bioreactors, where activities as high as 1630 U/ml were obtained. © Springer 2006.	en
heal.publisher	SPRINGER	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s11274-005-9063-7	en
dc.identifier.isi	ISI:000237468600014	en
dc.identifier.volume	22	en
dc.identifier.issue	5	en
dc.identifier.spage	501	en
dc.identifier.epage	506	en