Optimization of xylanase production by Sporotrichum thermophile using corn cobs and response surface methodology

Katapodis, P; Christakopoulou, V; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Christakopoulou, V	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:24:49Z
dc.date.available	2014-03-01T01:24:49Z
dc.date.issued	2006	en
dc.identifier.issn	1618-0240	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17451
dc.subject	Response Surface Methodology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Ammonium compounds	en
dc.subject.other	Bioreactors	en
dc.subject.other	Carbon	en
dc.subject.other	Crops	en
dc.subject.other	Fungi	en
dc.subject.other	Mathematical models	en
dc.subject.other	Nitrogen	en
dc.subject.other	Optimization	en
dc.subject.other	Sporotrichum thermophile	en
dc.subject.other	Surface methodology	en
dc.subject.other	Xylanase production	en
dc.subject.other	Enzymes	en
dc.subject.other	ammonia	en
dc.subject.other	carbon	en
dc.subject.other	nitrogen	en
dc.subject.other	phosphate	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	Ammonium compounds	en
dc.subject.other	Bioreactors	en
dc.subject.other	Carbon	en
dc.subject.other	Crops	en
dc.subject.other	Enzymes	en
dc.subject.other	Fungi	en
dc.subject.other	Mathematical models	en
dc.subject.other	Nitrogen	en
dc.subject.other	Optimization	en
dc.subject.other	bioreactor	en
dc.subject.other	carbon	en
dc.subject.other	experimental design	en
dc.subject.other	maize	en
dc.subject.other	nitrogen	en
dc.subject.other	optimization	en
dc.subject.other	article	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	corn	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	fungal strain	en
dc.subject.other	fungus culture	en
dc.subject.other	fungus growth	en
dc.subject.other	methodology	en
dc.subject.other	nonhuman	en
dc.subject.other	Sporothrix	en
dc.subject.other	sporotrichum thermophile	en
dc.subject.other	Corynascus heterothallicus	en
dc.subject.other	Zea mays	en
dc.title	Optimization of xylanase production by Sporotrichum thermophile using corn cobs and response surface methodology	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elsc.200520134	en
heal.identifier.secondary	http://dx.doi.org/10.1002/elsc.200520134	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	A 32 central composite experimental design was performed with the aim of optimizing the production of xylanase by Sporotrichum thermophile grown on corn cobs in submerged cultures. Various carbon and nitrogen sources were consecutively optimized, and corn cobs and ammonium phosphate concentrations were selected as substrates to test the effect of two variables, i.e., both substrate concentrations, on xylanase production. A second-order quadratic model and a response surface method showed that the optimum conditions for xylanase production were 2.7 % [w/ v] corn cobs and 0.7 % [w/v] ammonium phosphate. These optimum conditions were transferred to 7 L bioreactors, where activities as high as 56 U/mL were obtained. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.publisher	WILEY-V C H VERLAG GMBH	en
heal.journalName	Engineering in Life Sciences	en
dc.identifier.doi	10.1002/elsc.200520134	en
dc.identifier.isi	ISI:000240725500009	en
dc.identifier.volume	6	en
dc.identifier.issue	4	en
dc.identifier.spage	410	en
dc.identifier.epage	415	en