Characterization of a fungal strain isolated from a polyphenol polluted site

Dritsa, V; Rigas, F; Natsis, K; Marchant, R

dc.contributor.author	Dritsa, V	en
dc.contributor.author	Rigas, F	en
dc.contributor.author	Natsis, K	en
dc.contributor.author	Marchant, R	en
dc.date.accessioned	2014-03-01T01:26:00Z
dc.date.available	2014-03-01T01:26:00Z
dc.date.issued	2007	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17873
dc.subject	Basidiomycetes	en
dc.subject	Bioremediation	en
dc.subject	Biosorption	en
dc.subject	Biotransformation	en
dc.subject	PCR	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Basidiomycetes	en
dc.subject.other	Biosorption	en
dc.subject.other	Biotransformation	en
dc.subject.other	Polymerase chain reactions (PCR)	en
dc.subject.other	Bioremediation	en
dc.subject.other	Concentration (process)	en
dc.subject.other	DNA	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Genes	en
dc.subject.other	RNA	en
dc.subject.other	Soil pollution	en
dc.subject.other	Fungi	en
dc.subject.other	agar	en
dc.subject.other	aromatic compound	en
dc.subject.other	dye	en
dc.subject.other	internal transcribed spacer	en
dc.subject.other	laccase	en
dc.subject.other	lignin	en
dc.subject.other	lignin peroxidase	en
dc.subject.other	manganese peroxidase	en
dc.subject.other	olive oil	en
dc.subject.other	pentachlorophenol	en
dc.subject.other	polyphenol	en
dc.subject.other	polyporphyre	en
dc.subject.other	ribosome RNA	en
dc.subject.other	unclassified drug	en
dc.subject.other	Bioremediation	en
dc.subject.other	Concentration (process)	en
dc.subject.other	DNA	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Fungi	en
dc.subject.other	Genes	en
dc.subject.other	RNA	en
dc.subject.other	Soil pollution	en
dc.subject.other	adsorption	en
dc.subject.other	biodegradation	en
dc.subject.other	bioremediation	en
dc.subject.other	biotransformation	en
dc.subject.other	dye	en
dc.subject.other	enzyme activity	en
dc.subject.other	fungus	en
dc.subject.other	genetic analysis	en
dc.subject.other	growth rate	en
dc.subject.other	microbial activity	en
dc.subject.other	phenolic compound	en
dc.subject.other	pollutant removal	en
dc.subject.other	pollution tolerance	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	soil pollution	en
dc.subject.other	adsorption	en
dc.subject.other	article	en
dc.subject.other	base pairing	en
dc.subject.other	Basidiomycetes	en
dc.subject.other	biotransformation	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	Coprinellus xanthothrix	en
dc.subject.other	decolorization	en
dc.subject.other	DNA isolation	en
dc.subject.other	enzyme activity	en
dc.subject.other	fungal strain	en
dc.subject.other	fungus isolation	en
dc.subject.other	genome	en
dc.subject.other	Greece	en
dc.subject.other	growth rate	en
dc.subject.other	liquid culture	en
dc.subject.other	mycelial growth	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	priority journal	en
dc.subject.other	RNA transcription	en
dc.subject.other	soil pollution	en
dc.subject.other	Adsorption	en
dc.subject.other	Anthraquinones	en
dc.subject.other	Basidiomycota	en
dc.subject.other	Biodegradation, Environmental	en
dc.subject.other	Coloring Agents	en
dc.subject.other	Flavonoids	en
dc.subject.other	Kinetics	en
dc.subject.other	Laccase	en
dc.subject.other	Lignin	en
dc.subject.other	Peroxidases	en
dc.subject.other	Phenols	en
dc.subject.other	Polymerase Chain Reaction	en
dc.subject.other	Polymers	en
dc.subject.other	Soil Pollutants	en
dc.subject.other	Attica	en
dc.subject.other	Eurasia	en
dc.subject.other	Europe	en
dc.subject.other	Greece	en
dc.subject.other	Southern Europe	en
dc.subject.other	Basidiomycota	en
dc.subject.other	Coprinellus xanthothrix	en
dc.subject.other	Fungi	en
dc.title	Characterization of a fungal strain isolated from a polyphenol polluted site	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2006.07.025	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biortech.2006.07.025	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	A group of fungal strains were isolated from a polyphenol polluted soil, taken from an olive oil processing plant in Attica, Greece. The fungi were tested for their ability to decolorize a polyaromatic dye Poly R-478, which was used as a model compound to test their ligninolytic activities. The strain K1.1 decolorized efficiently the dye on agar plates and was further studied. PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA genes from the genomic DNA isolated from mycelium grown in liquid culture resulted in amplified fragments. Via BLASTN search, the length of a 773 base pairs was identified as the basidiomycetes Copri-nellus xanthothrix. The growth rates and the tolerance of the fungus were compared on solid media, containing four different concentrations of pentachlorophenol. Extracellular enzyme activities (lignin peroxidase, manganese peroxidase and laccase) were determined in defined liquid medium. The isolate expressed laccase and manganese peroxidase but not lignin peroxidase. The removal of the dye was also estimated in liquid medium. The fungus showed biosorption and biotransformation as removal mechanisms. (c) 2006 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Bioresource Technology	en
dc.identifier.doi	10.1016/j.biortech.2006.07.025	en
dc.identifier.isi	ISI:000244820900008	en
dc.identifier.volume	98	en
dc.identifier.issue	9	en
dc.identifier.spage	1741	en
dc.identifier.epage	1747	en