Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS

Tsimogiannis, D; Samiotaki, M; Panayotou, G; Oreopoulou, V

dc.contributor.author	Tsimogiannis, D	en
dc.contributor.author	Samiotaki, M	en
dc.contributor.author	Panayotou, G	en
dc.contributor.author	Oreopoulou, V	en
dc.date.accessioned	2014-03-01T01:26:01Z
dc.date.available	2014-03-01T01:26:01Z
dc.date.issued	2007	en
dc.identifier.issn	1420-3049	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/17874
dc.subject	Flavonoids	en
dc.subject	HPLC-MS/MS analysis	en
dc.subject	Origanum vulgare	en
dc.subject.classification	Chemistry, Organic	en
dc.subject.other	flavonoid	en
dc.subject.other	flavonol derivative	en
dc.subject.other	hydroxyl radical	en
dc.subject.other	plant extract	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	cyclization	en
dc.subject.other	high performance liquid chromatography	en
dc.subject.other	isolation and purification	en
dc.subject.other	mass spectrometry	en
dc.subject.other	oregano	en
dc.subject.other	time	en
dc.subject.other	ultraviolet spectrophotometry	en
dc.subject.other	Chromatography, High Pressure Liquid	en
dc.subject.other	Cyclization	en
dc.subject.other	Flavonoids	en
dc.subject.other	Flavonols	en
dc.subject.other	Hydroxyl Radical	en
dc.subject.other	Mass Spectrometry	en
dc.subject.other	Origanum	en
dc.subject.other	Plant Extracts	en
dc.subject.other	Spectrophotometry, Ultraviolet	en
dc.subject.other	Time Factors	en
dc.title	Characterization of flavonoid subgroups and hydroxy substitution by HPLC-MS/MS	en
heal.type	journalArticle	en
heal.identifier.primary	10.3390/12030593	en
heal.identifier.secondary	http://dx.doi.org/10.3390/12030593	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	HPLC-DAD coupled with mass spectrometry in the positive ionization mode was applied to study the fragmentation of twelve selected flavonoids. Compounds belonging to all the major subgroups found in common plants, i.e. flavonols, flavones, dihydroflavonols, flavanones and flavanols were studied. Compound standards were injected into the spectrometer and produced characteristic mass spectra. The fragmentation of each compound was studied and it was shown that the dehydration and carbon monoxide losses from the [M+H]+ ion by the members of each subgroup produced specific fragments, thus allowing the characterization of the flavonoid subgroups. Moreover, fragments resulting from fission of the C-rings are specific of each subgroup and revealed the substitution pattern of A- and B-rings. In order to verify the identifying efficiency of the positive ionization mode through these characteristic fragmentations, the unknown flavonoids of an Origanum vulgare diethyl ether extract were separated with the HPLC system and the major peaks were successfully identified with the mass spectrometer. © 2007 by MDPI.	en
heal.publisher	MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL	en
heal.journalName	Molecules	en
dc.identifier.doi	10.3390/12030593	en
dc.identifier.isi	ISI:000245437400029	en
dc.identifier.volume	12	en
dc.identifier.issue	3	en
dc.identifier.spage	593	en
dc.identifier.epage	606	en