Extraction and analysis of antioxidant components from Origanum dictamnus

Kouri, G; Tsimogiannis, D; Bardouki, H; Oreopoulou, V

dc.contributor.author	Kouri, G	en
dc.contributor.author	Tsimogiannis, D	en
dc.contributor.author	Bardouki, H	en
dc.contributor.author	Oreopoulou, V	en
dc.date.accessioned	2014-03-01T01:26:21Z
dc.date.available	2014-03-01T01:26:21Z
dc.date.issued	2007	en
dc.identifier.issn	1466-8564	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18025
dc.subject	DPPH radical	en
dc.subject	Flavonoids	en
dc.subject	Natural antioxidants	en
dc.subject	Oil oxidation	en
dc.subject	Origanum dictamnus	en
dc.subject.classification	Food Science & Technology	en
dc.subject.other	Cottonseed oil	en
dc.subject.other	Ethanol	en
dc.subject.other	Ethers	en
dc.subject.other	Oxidation	en
dc.subject.other	DPPH radical	en
dc.subject.other	Flavonoids	en
dc.subject.other	Natural antioxidants	en
dc.subject.other	Oil oxidation	en
dc.subject.other	Origanum dictamnus	en
dc.subject.other	Food additives	en
dc.subject.other	Dictamnus	en
dc.subject.other	Gossypium hirsutum	en
dc.subject.other	Micropus	en
dc.subject.other	Origanum	en
dc.subject.other	Origanum dictamnus	en
dc.title	Extraction and analysis of antioxidant components from Origanum dictamnus	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ifset.2006.09.003	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.ifset.2006.09.003	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	Three alternative extraction procedures were carried out in order to separate the antioxidant components and isolate an efficient extract from Origanum dictamnus. Procedure A included sequential extractions with petroleum ether (PE), diethyl ether (DE) and ethanol; procedure B sequential extractions with PE and ethyl acetate (EAc); procedure C a single step extraction with ethanol. The most efficient radical scavengers, according to the DPPH method, were isolated in ethanol extract of procedure A (mainly rosmarinic acid), followed by ethanol extract of procedure C. However, both ethanol extracts had low solubility in oil and could not protect it. EAc and DE extracts, containing mainly apigenin and epirosmanol ethyl ether, presented lower radical scavenging activity but were very effective against autoxidation of cottonseed oil (a concentration of 200 ppm was adequate to stabilize it). PE extract protected the oil effectively at concentration of 350 ppm, while being the least active against DPPH. Industrial relevance: This study examines Origanum dictamnus as a potential antioxidant additive for edible oils and fats. Three different extraction procedures of the raw material with non-toxic organic solvents were used to recover the sum of the extractable compounds and specific fractions respectively. The extraction procedures, accompanied with analytical techniques, led to the characterization of the antioxidant properties and the composition of the plant as well as the isolation of very efficient oil-soluble antioxidant fractions. © 2006 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Innovative Food Science and Emerging Technologies	en
dc.identifier.doi	10.1016/j.ifset.2006.09.003	en
dc.identifier.isi	ISI:000246424400001	en
dc.identifier.volume	8	en
dc.identifier.issue	2	en
dc.identifier.spage	155	en
dc.identifier.epage	162	en