Fluorescence and absorption assessment of a lipid mTHPC formulation following topical application in a non-melanotic skin tumor model

Johansson, A; Svensson, J; Bendsoe, N; Svanberg, K; Alexandratou, E; Kyriazi, M; Yova, D; Grafe, S; Trebst, T; Andersson-Engels, S

dc.contributor.author	Johansson, A	en
dc.contributor.author	Svensson, J	en
dc.contributor.author	Bendsoe, N	en
dc.contributor.author	Svanberg, K	en
dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Kyriazi, M	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Grafe, S	en
dc.contributor.author	Trebst, T	en
dc.contributor.author	Andersson-Engels, S	en
dc.date.accessioned	2014-03-01T01:26:22Z
dc.date.available	2014-03-01T01:26:22Z
dc.date.issued	2007	en
dc.identifier.issn	1083-3668	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18039
dc.subject	Absorption spectroscopy	en
dc.subject	Fluorescence imaging	en
dc.subject	mTHPC	en
dc.subject	Pharmacokinetics	en
dc.subject	Photodynamic therapy	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Optics	en
dc.subject.classification	Radiology, Nuclear Medicine & Medical Imaging	en
dc.subject.other	antineoplastic agent	en
dc.subject.other	drug carrier	en
dc.subject.other	lipid	en
dc.subject.other	liposome	en
dc.subject.other	mesoporphyrin	en
dc.subject.other	photosensitizing agent	en
dc.subject.other	temoporfin	en
dc.subject.other	animal	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	disease model	en
dc.subject.other	fluorescence microscopy	en
dc.subject.other	hairless mouse	en
dc.subject.other	male	en
dc.subject.other	melanoma	en
dc.subject.other	metabolism	en
dc.subject.other	methodology	en
dc.subject.other	mouse	en
dc.subject.other	pathology	en
dc.subject.other	photochemotherapy	en
dc.subject.other	skin absorption	en
dc.subject.other	skin tumor	en
dc.subject.other	topical drug administration	en
dc.subject.other	treatment outcome	en
dc.subject.other	Administration, Topical	en
dc.subject.other	Animals	en
dc.subject.other	Antineoplastic Agents	en
dc.subject.other	Disease Models, Animal	en
dc.subject.other	Drug Carriers	en
dc.subject.other	Lipids	en
dc.subject.other	Liposomes	en
dc.subject.other	Male	en
dc.subject.other	Melanoma	en
dc.subject.other	Mesoporphyrins	en
dc.subject.other	Mice	en
dc.subject.other	Mice, Inbred HRS	en
dc.subject.other	Microscopy, Fluorescence	en
dc.subject.other	Photochemotherapy	en
dc.subject.other	Photosensitizing Agents	en
dc.subject.other	Skin Absorption	en
dc.subject.other	Skin Neoplasms	en
dc.subject.other	Treatment Outcome	en
dc.subject.other	Absorption spectroscopy	en
dc.subject.other	Fluorescence	en
dc.subject.other	Liposomes	en
dc.subject.other	Pharmacokinetics	en
dc.subject.other	Photosensitivity	en
dc.subject.other	Photosensitizers	en
dc.subject.other	Porphyrins	en
dc.subject.other	Skin	en
dc.subject.other	Tumors	en
dc.subject.other	Meso-tetra(hydroxyphenyl)chlorin	en
dc.subject.other	Non-melanotic skin tumor model	en
dc.subject.other	Photodynamic therapy	en
dc.subject.other	Murinae	en
dc.subject.other	Tetra	en
dc.title	Fluorescence and absorption assessment of a lipid mTHPC formulation following topical application in a non-melanotic skin tumor model	en
heal.type	journalArticle	en
heal.identifier.primary	10.1117/1.2743080	en
heal.identifier.secondary	http://dx.doi.org/10.1117/1.2743080	en
heal.identifier.secondary	034026	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	Although the benefits of topical sensitizer administration have been confirmed for photodynamic therapy (PDT) ALA-induced, protoporphyrin IX is the only sensitizer clinically used with this administration route. Unfortunately, ALA-PDT results in poor treatment response for thicker lesions. Here, selectivity and depth distribution of the highly potent sensitizer meso-tetra(hydroxyphenyl)chlorin (mTHPC), supplied in a novel liposome formulation was investigated following topical administration for 4 and 6 h in a murine skin tumor model. Extraction data indicated an average [standard deviation (SD)] mTHPC concentration within lesions of 6.0(+/- 3.1) ng/mg tissue with no significant difference (p<0.05) between 4- and 6-h application times and undetectable levels of generalized photosensitivity. Absorption spectroscopy and chemical extraction both indicated a significant selectivity between lesion and normal surrounding skin at 4 and 6 h, whereas the more sensitive fluorescence imaging setup revealed significant selectivity only for the 4-h application time. Absorption data showed a significant correlation with extraction, whereas the results from the fluorescence imaging setup did not correlate with the other methods. Our results indicate that this sensitizer formulation and administration path could be interesting for topical mTHPC-PDT, decreasing the effects of extended skin photosensitivity associated with systemic mTHPC administration. (C) 2007 Society of Photo-Optical instrumentation Engineers.	en
heal.publisher	SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS	en
heal.journalName	Journal of Biomedical Optics	en
dc.identifier.doi	10.1117/1.2743080	en
dc.identifier.isi	ISI:000248504500030	en
dc.identifier.volume	12	en
dc.identifier.issue	3	en