In vitro three-stage continuous fermentation of wheat arabinoxylan fractions and induction of hydrolase activity by the gut microflora

Vardakou, M; Nueno Palop, C; Gasson, M; Narbad, A; Christakopoulos, P

dc.contributor.author	Vardakou, M	en
dc.contributor.author	Nueno Palop, C	en
dc.contributor.author	Gasson, M	en
dc.contributor.author	Narbad, A	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:26:28Z
dc.date.available	2014-03-01T01:26:28Z
dc.date.issued	2007	en
dc.identifier.issn	0141-8130	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18089
dc.subject	Arabinoxylan	en
dc.subject	Ferulic acid esterases	en
dc.subject	Three-stage in vitro colon model	en
dc.subject	Xylanases	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	arabinoxylan	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid	en
dc.subject.other	hydrolase	en
dc.subject.other	prebiotic agent	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	article	en
dc.subject.other	bacterial count	en
dc.subject.other	bacterial metabolism	en
dc.subject.other	Bacteroides	en
dc.subject.other	Bifidobacterium	en
dc.subject.other	Clostridium	en
dc.subject.other	continuous fermentation	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme induction	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	intestine flora	en
dc.subject.other	nonhuman	en
dc.subject.other	wheat	en
dc.subject.other	DNA Primers	en
dc.subject.other	DNA, Ribosomal	en
dc.subject.other	Endo-1,4-beta Xylanases	en
dc.subject.other	Fermentation	en
dc.subject.other	Flour	en
dc.subject.other	Humans	en
dc.subject.other	Hydrolases	en
dc.subject.other	Intestinal Mucosa	en
dc.subject.other	Intestine, Large	en
dc.subject.other	Kinetics	en
dc.subject.other	RNA, Ribosomal, 16S	en
dc.subject.other	Triticum	en
dc.subject.other	Xylans	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Bacteroides sp.	en
dc.subject.other	Clostridium	en
dc.subject.other	Triticum aestivum	en
dc.title	In vitro three-stage continuous fermentation of wheat arabinoxylan fractions and induction of hydrolase activity by the gut microflora	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijbiomac.2007.07.017	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.ijbiomac.2007.07.017	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	In vitro fermentations were carried out by using a model of the human colon to stimulate microbial activities of gut bacteria. The model consisted of a three-stage culture system. Bacterial populations were evaluated under the effect of three types of arabinoxylan, a nonstarch polysaccharide, derived from wheat, the water-unextractable arabinoxylan fraction (WU-AX), WU-AX pretreated with exogenous xylanase and the soluble water-extractable arabinoxylan fraction (WE-AX). The xylanase pretreated (WU-AX) had a stimulatory effect upon colonic bifidobacteria throughout all three vessels. Counts of Bacteroides spp. and Clostridium spp. were also both significantly reduced. Addition of the WU-AX substrates to the first vessel resulted in induction of bacteria] synthesis of extracellular hydrolytic enzymes xylanase and ferulic acid esterase which are both required for bacterial metabolism of WU-AX; this induction was significantly greater with the xylanase treated WU-AX. (C) 2007 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	International Journal of Biological Macromolecules	en
dc.identifier.doi	10.1016/j.ijbiomac.2007.07.017	en
dc.identifier.isi	ISI:000251068800013	en
dc.identifier.volume	41	en
dc.identifier.issue	5	en
dc.identifier.spage	584	en
dc.identifier.epage	589	en