Investigation of the chromatographic behaviour of some selenium species-Comparison with their octanol-water partitioning

Tsopelas, F; Tsantili-Kakoulidou, A; Ochsenkuhn-Petropoulou, M

dc.contributor.author	Tsopelas, F	en
dc.contributor.author	Tsantili-Kakoulidou, A	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.date.accessioned	2014-03-01T01:26:31Z
dc.date.available	2014-03-01T01:26:31Z
dc.date.issued	2007	en
dc.identifier.issn	0039-9140	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18110
dc.subject	Extrapolated retention factors log kw	en
dc.subject	Lipophilicity	en
dc.subject	Logarithm of distribution coefficient log D	en
dc.subject	Mobile phase additives	en
dc.subject	Reversed-phase liquid chromatography	en
dc.subject	Selenium speciation	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Extrapolated retention factors	en
dc.subject.other	Lipophilicity	en
dc.subject.other	Mobile phase additives	en
dc.subject.other	Reversed-phase liquid chromatography	en
dc.subject.other	Selenium speciation	en
dc.subject.other	Column chromatography	en
dc.subject.other	Distribution functions	en
dc.subject.other	Liquid chromatography	en
dc.subject.other	Methanol	en
dc.subject.other	Phase behavior	en
dc.subject.other	Selenium compounds	en
dc.title	Investigation of the chromatographic behaviour of some selenium species-Comparison with their octanol-water partitioning	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.talanta.2007.03.006	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.talanta.2007.03.006	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	The retention behaviour of selenites (Se(IV)), selenates (Se(Vl)), seleno-DL-methionine (Se-Met), selenocystine (Se-Cyst), selenocystamine (Se-CM) and selenourea (Se-U) was investigated using a Discovery end-capped reversed-phase column as stationary phase and different mobile phase conditions. Extrapolated to 100% aqueous mobile phase retention factors (log kw) of the investigated Se species, determined using different methanol fractions (phi) as organic modifier, were compared with the corresponding actual values. The proper operation of this column even at 100% aqueous phase proved to be valuable for the accurate determination of log k(w) values of Se-CM and Se-Cyst, presenting a convex curvature log k =f(phi)at low MeOH fractions, often neglected in the extrapolation procedure. The effect of the presence of n-decylamine as well as saturation of the mobile phase with n-octanol was also studied. For ampholytic Se-Met and Se-Cyst the effect of n-decylamine in retention reflected the predominance of zwitterionic nature in the case of Se-Met in contrary to the non-zwitterionic species found in the case of Se-Cyst, in accordance with our previous findings concerning partitioning experiments in the n-octanol/water system. Finally, an attempt was made to correlate log k, values with the logarithm of n-octanol/water distribution coefficient, log D, of the investigated Se species and an indicative log D value of Se-U was derived. (c) 2007 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Talanta	en
dc.identifier.doi	10.1016/j.talanta.2007.03.006	en
dc.identifier.isi	ISI:000249174700017	en
dc.identifier.volume	73	en
dc.identifier.issue	1	en
dc.identifier.spage	127	en
dc.identifier.epage	133	en