Manganese speciation in human cerebrospinal fluid using CZE coupled to inductively coupled plasma MS

Michalke, B; Berthele, A; Mistriotis, P; Ochsenkuhn-Petropoulou, M; Halbach, S

dc.contributor.author	Michalke, B	en
dc.contributor.author	Berthele, A	en
dc.contributor.author	Mistriotis, P	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.contributor.author	Halbach, S	en
dc.date.accessioned	2014-03-01T01:26:34Z
dc.date.available	2014-03-01T01:26:34Z
dc.date.issued	2007	en
dc.identifier.issn	0173-0835	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18136
dc.subject	Cerebrospinal fluid	en
dc.subject	Coupling	en
dc.subject	CZE	en
dc.subject	Dynamic reaction cell	en
dc.subject	Inductively coupled plasma MS	en
dc.subject	Manganese	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	2 oxoglutaric acid	en
dc.subject.other	adenosine	en
dc.subject.other	citric acid	en
dc.subject.other	fumaric acid	en
dc.subject.other	histidine	en
dc.subject.other	malic acid	en
dc.subject.other	manganese	en
dc.subject.other	nicotinamide adenine dinucleotide	en
dc.subject.other	oxaloacetic acid	en
dc.subject.other	article	en
dc.subject.other	blood brain barrier	en
dc.subject.other	capillary zone electrophoresis	en
dc.subject.other	cerebrospinal fluid analysis	en
dc.subject.other	cerebrospinal fluid level	en
dc.subject.other	citric acid cycle	en
dc.subject.other	human	en
dc.subject.other	mass spectrometry	en
dc.subject.other	metal metabolism	en
dc.subject.other	ultraviolet radiation	en
dc.subject.other	Citrates	en
dc.subject.other	Electrophoresis, Capillary	en
dc.subject.other	Humans	en
dc.subject.other	Manganese	en
dc.subject.other	Manganese Compounds	en
dc.subject.other	Mass Spectrometry	en
dc.subject.other	Reference Standards	en
dc.subject.other	Reproducibility of Results	en
dc.title	Manganese speciation in human cerebrospinal fluid using CZE coupled to inductively coupled plasma MS	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elps.200600686	en
heal.identifier.secondary	http://dx.doi.org/10.1002/elps.200600686	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	The neurotoxic effects of manganese (Mn) at elevated concentrations are well known. This raises the question, which of the Mn species can cross neural barriers and appear in cerebrospinal fluid (CSF). CSF is the last matrix in a living human organism available for analysis before a compound reaches the brain cells and therefore it is assumed to reflect best the internal exposure of brain tissue to Mn species. A previously developed CE method was modified for separation of albumin, histidine, tyrosine, cystine, fumarate, malate, inorganic Mn, oxalacetate, α-keto-glutarate, nicotinamide-dinucleotide (NAD), citrate, adenosine, glutathione, and glutamine. These compounds are supposed in the literature to act as potential Mn carriers. In a first attempt, these compounds were analyzed by CZE-UV to check whether they are present in CSF. The CZE-UV method was simpler than the coupled CZE-inductively coupled plasma (ICP)-dynamic reaction cell (DRC)-MS method and it was therefore chosen to obtain a first overview information. In a second step, the coupled method (CZE-ICP-DRC-MS) was used to analyze, in detail, which of the compounds found in CSF by CZE-UV were actually bound to Mn. Finally, 13 Mn species were monitored in CSF samples, most of them being identified: Mn-histidine, Mn-fumarate, Mn-malate, inorganic Mn, Mn-oxalacetate, Mn-α-keto glutarate, Mn-carrying NAD, Mn-citrate and Mn-adenosine. By far the most abundant Mn species was Mn-citrate showing a concentration of 0.7 ± 0.13 μg Mn/L. Interestingly, several other Mn species can be related to the citric acid cycle. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.publisher	WILEY-V C H VERLAG GMBH	en
heal.journalName	Electrophoresis	en
dc.identifier.doi	10.1002/elps.200600686	en
dc.identifier.isi	ISI:000246557400011	en
dc.identifier.volume	28	en
dc.identifier.issue	9	en
dc.identifier.spage	1380	en
dc.identifier.epage	1386	en