Manganese species from human serum, cerebrospinal fluid analyzed by size exclusion chromatography-, capillary electrophoresis coupled to inductively coupled plasma mass spectrometry

Michalke, B; Berthele, A; Mistriotis, P; Ochsenkuhn-Petropoulou, M; Halbach, S

dc.contributor.author	Michalke, B	en
dc.contributor.author	Berthele, A	en
dc.contributor.author	Mistriotis, P	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.contributor.author	Halbach, S	en
dc.date.accessioned	2014-03-01T01:26:34Z
dc.date.available	2014-03-01T01:26:34Z
dc.date.issued	2007	en
dc.identifier.issn	0946672X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18137
dc.subject	Cerebrospinal fluid	en
dc.subject	Inductively coupled plasma mass spectrometry (ICP-MS)	en
dc.subject	Mn-speciation	en
dc.subject	Serum	en
dc.subject	Size exclusion chromatography (SEC)	en
dc.subject.other	manganese	en
dc.subject.other	manganese citrate	en
dc.subject.other	unclassified drug	en
dc.subject.other	article	en
dc.subject.other	atomic emission spectrometry	en
dc.subject.other	blood analysis	en
dc.subject.other	blood level	en
dc.subject.other	capillary electrophoresis	en
dc.subject.other	cerebrospinal fluid analysis	en
dc.subject.other	complex formation	en
dc.subject.other	gel permeation chromatography	en
dc.subject.other	human	en
dc.subject.other	human tissue	en
dc.subject.other	particle size	en
dc.subject.other	priority journal	en
dc.subject.other	Chromatography, Gel	en
dc.subject.other	Electrophoresis, Capillary	en
dc.subject.other	Humans	en
dc.subject.other	Manganese	en
dc.subject.other	Mass Spectrometry	en
dc.title	Manganese species from human serum, cerebrospinal fluid analyzed by size exclusion chromatography-, capillary electrophoresis coupled to inductively coupled plasma mass spectrometry	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jtemb.2007.09.004	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.jtemb.2007.09.004	en
heal.publicationDate	2007	en
heal.abstract	Manganese (Mn) at high concentrations can have adverse effects on health, mainly because of its toxicity to the central nervous system. Health impacts of Mn are known mostly from occupational health studies, but the exact mechanisms how Mn, being bound to transferrin (TF) in the blood, enters the brain - are unknown. Mn speciation at the neural barriers can help to obtain more information about the pathways and carriers. This paper summarizes investigations on the size distribution of Mn carriers (e.g. proteins, peptides, carbonic acids) in serum before the neural barriers and in cerebrospinal fluid (CSF) behind them as a first characterization step of the Mn carriers being involved in moving Mn across the neural barriers. Further identification of Mn-species in CSF was successfully achieved by CZE-inductively coupled plasma (ICP)-dynamic reaction cell (DRC)-mass spectrometry (MS). Serum samples showed Mn mean concentrations of 1.7±0.8 μg L-1. The size distribution of Mn-carriers showed a main peak in the TF/albumin size fitting to the known physiological ligands. However, also an increasing Mn peak at 700 Da with increasing total Mn concentration was seen. Samples of CSF showed Mn mean concentrations of 2.6 μg L-1=48 nM. In CSF Mn was found to be mostly bound to low-molecular-mass (LMM)-Mn carriers in the range of 640-680 Da. This is similar to the LMM compound in serum and to Mn-citrate complexes suggested to be present in body fluids. Citrate concentration was 573 μM, thus being in huge excess compared to Mn. CSF was further analyzed by CZE-ICP-DRC-MS. Several Mn-species were monitored and mostly identified. The most abundant Mn-species was Mn-citrate at a concentration of around 0.7 μg Mn L-1. © 2007 Elsevier GmbH. All rights reserved.	en
heal.journalName	Journal of Trace Elements in Medicine and Biology	en
dc.identifier.doi	10.1016/j.jtemb.2007.09.004	en
dc.identifier.volume	21	en
dc.identifier.issue	SUPPL. 1	en
dc.identifier.spage	4	en
dc.identifier.epage	9	en