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Passive optical separation within a 'nondiffracting' light beam

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dc.contributor.author Paterson, L en
dc.contributor.author Papagiakoumou, E en
dc.contributor.author Milne, G en
dc.contributor.author Garcs-Chavez, V en
dc.contributor.author Briscoe, T en
dc.contributor.author Sibbett, W en
dc.contributor.author Dholakia, K en
dc.contributor.author Riches ,AC en
dc.date.accessioned 2014-03-01T01:26:52Z
dc.date.available 2014-03-01T01:26:52Z
dc.date.issued 2007 en
dc.identifier.issn 1083-3668 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/18267
dc.subject Bessel beam en
dc.subject Cell sorting en
dc.subject Optics en
dc.subject Stem cells en
dc.subject.classification Biochemical Research Methods en
dc.subject.classification Optics en
dc.subject.classification Radiology, Nuclear Medicine & Medical Imaging en
dc.subject.other Bone en
dc.subject.other Light en
dc.subject.other Microspheres en
dc.subject.other Particle size en
dc.subject.other Refractive index en
dc.subject.other Bessel beams en
dc.subject.other Cell sorting en
dc.subject.other Cell culture en
dc.subject.other Murinae en
dc.subject.other animal en
dc.subject.other article en
dc.subject.other cell separation en
dc.subject.other human en
dc.subject.other laboratory diagnosis en
dc.subject.other methodology en
dc.subject.other microdissection en
dc.subject.other optical tweezers en
dc.subject.other refractometry en
dc.subject.other Animals en
dc.subject.other Cell Separation en
dc.subject.other Humans en
dc.subject.other Microdissection en
dc.subject.other Optical Tweezers en
dc.subject.other Refractometry en
dc.subject.other Specimen Handling en
dc.title Passive optical separation within a 'nondiffracting' light beam en
heal.type journalArticle en
heal.identifier.primary 10.1117/1.2794780 en
heal.identifier.secondary http://dx.doi.org/10.1117/1.2794780 en
heal.identifier.secondary 054017 en
heal.language English en
heal.publicationDate 2007 en
heal.abstract A passive, optical cell sorter is created using the light pattern of a 'nondiffracting' beam - the Bessel beam. As a precursor to cell sorting studies, microspheres are used to test the resolution of the sorter on the basis of particle size and refractive index. Variations in size and, more noticeably, refractive index, lead to a marked difference in the migration time of spheres in the Bessel beam. Intrinsic differences (size, refractive index) between native (unlabeled) cell populations are utilized for cell sorting. The large difference in size between erythrocytes and lymphocytes results in their successful separation in this beam pattern. The intrinsic differences in size and refractive index of other cells in the study (HL60 human promyelocytic leukaemic cells, murine bone marrow, and murine stem/progenitor cells) are not large enough to induce passive optical separation. Silica microsphere tags are attached to cells of interest to modify their size and refractive index, resulting in the separation of labeled cells. Cells collected after separation are viable, as evidenced by trypan blue dye exclusion, their ability to clone in vitro, continued growth in culture, and lack of expression of Caspase 3, a marker of apoptosis. (C) 2007 Society of Photo-Optical Instrumentation Engineers. en
heal.publisher SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS en
heal.journalName Journal of Biomedical Optics en
dc.identifier.doi 10.1117/1.2794780 en
dc.identifier.isi ISI:000251549600048 en
dc.identifier.volume 12 en
dc.identifier.issue 5 en


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