Characterization, morphology and composition of biofilm and precipitates from a sulphate-reducing fixed-bed reactor

Remoundaki, E; Kousi, P; Joulian, C; Battaglia-Brunet, F; Hatzikioseyian, A; Tsezos, M

dc.contributor.author	Remoundaki, E	en
dc.contributor.author	Kousi, P	en
dc.contributor.author	Joulian, C	en
dc.contributor.author	Battaglia-Brunet, F	en
dc.contributor.author	Hatzikioseyian, A	en
dc.contributor.author	Tsezos, M	en
dc.date.accessioned	2014-03-01T01:28:02Z
dc.date.available	2014-03-01T01:28:02Z
dc.date.issued	2008	en
dc.identifier.issn	0304-3894	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18675
dc.subject	Amorphous phases	en
dc.subject	dsrAB gene diversity	en
dc.subject	Fixed-bed reactor	en
dc.subject	Iron sulphides	en
dc.subject	Sulphate-reducing bacteria	en
dc.subject	Zinc sulphides	en
dc.subject.classification	Engineering, Environmental	en
dc.subject.classification	Engineering, Civil	en
dc.subject.classification	Environmental Sciences	en
dc.subject.other	Amorphous phases	en
dc.subject.other	Fixed-bed reactor	en
dc.subject.other	Sulphate-reducing bacteria	en
dc.subject.other	Chemical reactors	en
dc.subject.other	Energy dispersive spectroscopy	en
dc.subject.other	Scanning electron microscopy	en
dc.subject.other	X ray diffraction analysis	en
dc.subject.other	Biofilms	en
dc.subject.other	ferrous sulfide	en
dc.subject.other	iron	en
dc.subject.other	marcasite	en
dc.subject.other	sulfate	en
dc.subject.other	unclassified drug	en
dc.subject.other	wurtzite	en
dc.subject.other	zinc	en
dc.subject.other	zinc sulfide	en
dc.subject.other	Biofilms	en
dc.subject.other	Chemical reactors	en
dc.subject.other	Energy dispersive spectroscopy	en
dc.subject.other	Scanning electron microscopy	en
dc.subject.other	X ray diffraction analysis	en
dc.subject.other	bacterium	en
dc.subject.other	biofilm	en
dc.subject.other	bioreactor	en
dc.subject.other	gene	en
dc.subject.other	iron sulfide	en
dc.subject.other	mineralogy	en
dc.subject.other	morphology	en
dc.subject.other	scanning electron microscopy	en
dc.subject.other	sulfate-reducing bacterium	en
dc.subject.other	X-ray diffraction	en
dc.subject.other	zinc	en
dc.subject.other	article	en
dc.subject.other	bacterial cell	en
dc.subject.other	biofilm	en
dc.subject.other	chemical analysis	en
dc.subject.other	fixed bed reactor	en
dc.subject.other	particle size	en
dc.subject.other	pH	en
dc.subject.other	Proteobacteria	en
dc.subject.other	scanning electron microscopy	en
dc.subject.other	X ray	en
dc.subject.other	X ray diffraction	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Biofilms	en
dc.subject.other	Bioreactors	en
dc.subject.other	Ferrous Compounds	en
dc.subject.other	Genes, Bacterial	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Precipitation	en
dc.subject.other	RNA, Ribosomal, 16S	en
dc.subject.other	Sequence Alignment	en
dc.subject.other	Sequence Analysis, DNA	en
dc.subject.other	Sulfates	en
dc.subject.other	Sulfides	en
dc.subject.other	Sulfur-Reducing Bacteria	en
dc.subject.other	Waste Disposal, Fluid	en
dc.subject.other	Water Pollutants, Chemical	en
dc.subject.other	Zinc Compounds	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Deltaproteobacteria	en
dc.title	Characterization, morphology and composition of biofilm and precipitates from a sulphate-reducing fixed-bed reactor	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jhazmat.2007.08.094	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.jhazmat.2007.08.094	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	The characteristics of the biofilm and the solids formed during the operation of a sulphate-reducing fixed-bed reactor, fed with a moderately acidic synthetic effluent containing zinc and iron, are presented. A diverse population of delta-Proteobacteria SRB, affiliated to four distinct genera, colonized the system. The morphology, mineralogy and surface chemistry of the precipitates were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The XRD patterns observed are characteristic of amorphous solid phases. Peaks corresponding to crystalline iron sulphide, marcasite, sphalerite and wurtzite were also identified. SEM-EDX results confirm the predominance of amorphous phases appearing as a cloudy haze. EDX spectra of spots on the surface of these amorphous phases reveal the predominance of iron, zinc and sulphur indicating the formation of iron and zinc sulphides. The predominance of these amorphous phases and the formation of very fine particles, during the operation of the SRB column, are in agreement and can be explained by the formation pathways of metal sulphides at ambient temperature, alkaline pH and reducing conditions. Solids are precipitated either as (i) amorphous phases deposited on the bed material, as well as on surface of crystals, e.g. Mg-3(PO4)(2) and (ii) as rod-shaped solids characterized by a rough hazy surface, indicating the encapsulation of bacterial cells by amorphous metal sulphides. (c) 2007 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Hazardous Materials	en
dc.identifier.doi	10.1016/j.jhazmat.2007.08.094	en
dc.identifier.isi	ISI:000255417500064	en
dc.identifier.volume	153	en
dc.identifier.issue	1-2	en
dc.identifier.spage	514	en
dc.identifier.epage	524	en