Enzymic production of feruloyl xylo-oligosaccharides from corn cobs by a family 10 xylanase from Thermoascus aurantiacus

Katapodis, P; Christakopoulos, P

dc.contributor.author	Katapodis, P	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:28:16Z
dc.date.available	2014-03-01T01:28:16Z
dc.date.issued	2008	en
dc.identifier.issn	0023-6438	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18796
dc.subject	Autoclave treatment	en
dc.subject	Corn cobs	en
dc.subject	Feruloyl xylo-oligosaccharides	en
dc.subject	Sonication	en
dc.subject	Xylanase	en
dc.subject.classification	Food Science & Technology	en
dc.subject.other	Enzyme activity	en
dc.subject.other	Food products	en
dc.subject.other	Kinetic parameters	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Sonication	en
dc.subject.other	Autoclave treatment	en
dc.subject.other	Corn cobs (CCs)	en
dc.subject.other	Feruloyl xylo-oligosaccharides	en
dc.subject.other	Xylanase	en
dc.subject.other	Oligosaccharides	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.subject.other	Zea mays	en
dc.title	Enzymic production of feruloyl xylo-oligosaccharides from corn cobs by a family 10 xylanase from Thermoascus aurantiacus	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.lwt.2007.08.004	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.lwt.2007.08.004	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Feruloyl xylo-oligosaccharides (FeXOSs) were obtained from corn cobs (CCs) by treatment with a Thermoascus aurantiacus family beta-D-endoxylanase. CCs subjected to a sonication (ST-CC) or an autoclave treatment in order to enhance enzymic access and FeXOS production. Enzymic FeXOS production was increased by CCs thermal treatment up to 3.5 times. The kinetic parameters K-E and V-max, describing enzyme-dependent production rates of FeXOSs from CCs, were estimated at 130 +/- 20 nM and 290 +/- 10 nM h(-1), respectively. The reaction parameters affecting the FeXOS production, such as substrate concentration, enzyme loading and reaction time, have been investigated. The maximum FeXOS production was observed after 24h incubation of 100 g L-1 AT-CC with 570 nM xylanase. The obtained yield was 12 mu mol of FeXOSs per gram of CC. (c) 2007 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	LWT - Food Science and Technology	en
dc.identifier.doi	10.1016/j.lwt.2007.08.004	en
dc.identifier.isi	ISI:000256407900012	en
dc.identifier.volume	41	en
dc.identifier.issue	7	en
dc.identifier.spage	1239	en
dc.identifier.epage	1243	en