Evaluation of Fusarium oxysporum as an enzyme factory for the hydrolysis of brewer's spent grain with improved biodegradability for ethanol production

Xiros, C; Topakas, E; Katapodis, P; Christakopoulos, P

dc.contributor.author	Xiros, C	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:28:18Z
dc.date.available	2014-03-01T01:28:18Z
dc.date.issued	2008	en
dc.identifier.issn	0926-6690	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18804
dc.subject	brewers spent grain	en
dc.subject	alkali pretreatment	en
dc.subject	lignocellulolytic enzymes	en
dc.subject	solid-state culture	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Agronomy	en
dc.subject.other	SOLID-STATE FERMENTATION	en
dc.subject.other	ALPHA-L-ARABINOFURANOSIDASES	en
dc.subject.other	WHEAT-STRAW	en
dc.subject.other	FERULOYL ESTERASES	en
dc.subject.other	THERMOASCUS-AURANTIACUS	en
dc.subject.other	SUBSTRATE FERMENTATION	en
dc.subject.other	ALKALINE-HYDROLYSIS	en
dc.subject.other	XYLANASE PRODUCTION	en
dc.subject.other	NEUROSPORA-CRASSA	en
dc.subject.other	DIRECT CONVERSION	en
dc.title	Evaluation of Fusarium oxysporum as an enzyme factory for the hydrolysis of brewer's spent grain with improved biodegradability for ethanol production	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.indcrop.2008.02.004	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.indcrop.2008.02.004	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Brewer's spent grain (BG), the most abundant brewing by-product, is used in the present study as a low-cost feedstock for the production of ethanol by the mesophilic fungus Fusarium oxysporum using a consolidated bioconversion process. The production of required cellulolytic and hemicellulolytic enzymes was optimized under solid-state cultivation (SSC) concerning carbon source and initial moisture. The optimal medium contains BG and corn cobs (CC) in a ratio 7:3 while the optimal initial moisture is 66% (w/w). SSC in a laboratory horizontal bioreactor using the optimized medium allowed the large-scale production of a multienzymic system including endoglucanase, Cellobiohydrolase, P-D-glucosidase, xylanase, feruloyl esterase, acetyl esterase, P-D-xylosidase and a-L-arabinofuranosidase. Chromogenic (fluorogenic) 4-methylumbelliferyl substrates were used to partially characterize the extracellular proteome of the microbe after the separation by isolectzic focusing (IEF) electrophoresis. Alkali pretreatment of brewer's spent grain and different aeration levels were studied for the optimization of the ethanol production by F. oxysporum in a consecutive submerged fermentation. A yield about 65 g ethanol kg(-1) of dry BG was obtained with alkali pretreated BG under microaerobic conditions (0.01 vvm) corresponding to 30% of the theoretical yield based on total glucose and xylose composition of BG. (C) 2008 Elsevier B.V All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	INDUSTRIAL CROPS AND PRODUCTS	en
dc.identifier.doi	10.1016/j.indcrop.2008.02.004	en
dc.identifier.isi	ISI:000258987800013	en
dc.identifier.volume	28	en
dc.identifier.issue	2	en
dc.identifier.spage	213	en
dc.identifier.epage	224	en