Evaluation of the prebiotic properties of wheat arabinoxylan fractions and induction of hydrolase activity in gut microflora

Vardakou, M; Palop, CN; Christakopoulos, P; Faulds, CB; Gasson, MA; Narbad, A

dc.contributor.author	Vardakou, M	en
dc.contributor.author	Palop, CN	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Faulds, CB	en
dc.contributor.author	Gasson, MA	en
dc.contributor.author	Narbad, A	en
dc.date.accessioned	2014-03-01T01:28:21Z
dc.date.available	2014-03-01T01:28:21Z
dc.date.issued	2008	en
dc.identifier.issn	0168-1605	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18807
dc.subject	Arabinoxylan	en
dc.subject	Ferulic acid esterase	en
dc.subject	Gut bacteria	en
dc.subject	Prebiotic	en
dc.subject	Xylanase	en
dc.subject.classification	Food Science & Technology	en
dc.subject.classification	Microbiology	en
dc.subject.other	arabinoxylan	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid esterase	en
dc.subject.other	hydrolase	en
dc.subject.other	prebiotic agent	en
dc.subject.other	unclassified drug	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	anaerobic fermentation	en
dc.subject.other	article	en
dc.subject.other	bacterial metabolism	en
dc.subject.other	Bifidobacterium	en
dc.subject.other	Clostridium	en
dc.subject.other	controlled study	en
dc.subject.other	Enterobacter	en
dc.subject.other	enzyme activity	en
dc.subject.other	hydrolysis	en
dc.subject.other	intestine flora	en
dc.subject.other	Lactobacillus	en
dc.subject.other	nonhuman	en
dc.subject.other	Bifidobacterium	en
dc.subject.other	Carboxylic Ester Hydrolases	en
dc.subject.other	Colony Count, Microbial	en
dc.subject.other	Endo-1,4-beta Xylanases	en
dc.subject.other	Fermentation	en
dc.subject.other	Humans	en
dc.subject.other	Intestine, Large	en
dc.subject.other	Kinetics	en
dc.subject.other	Lactobacillus	en
dc.subject.other	Probiotics	en
dc.subject.other	Triticum	en
dc.subject.other	Xylans	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Triticum aestivum	en
dc.title	Evaluation of the prebiotic properties of wheat arabinoxylan fractions and induction of hydrolase activity in gut microflora	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijfoodmicro.2007.11.007	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.ijfoodmicro.2007.11.007	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Dietary supplementation with prebiotics may result in the stimulation of the growth of beneficial bacteria such as lactobacilli and bifidobacteria in the human gastrointestinal tract. The effect of water-unextractable arabinoxylans (WU-AX) derived from wheat on the modulation of gut bacterial composition was investigated using a mixed culture fermentation system. A prebiotic index (PI) score of 2.03 was obtained after addition of 1% (w/v) WU-AX to a pH-controlled stirred anaerobic fermentation vessel. Pretreatment of the WU-AX with endo-beta-1,4-xylanase resulted in significantly higher PI value (3.48) indicating that pretreatment provided oligomers; that were better utilised by the gut bacteria. The extracellular hydrolytic enzymes xylanase and ferulic acid esterase are both required for bacterial metabolism of WU-AX and both activities were present in supernatants derived from the mixed batch cultures. Addition of the WU-AX substrates to the batch cultures produced several fold increases of bacterial synthesis of both enzymes, and these increases were greater when the WU-AX substrate was pretreated with xylanase. (C) 2007 Elsevier B.V. All fights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.doi	10.1016/j.ijfoodmicro.2007.11.007	en
dc.identifier.isi	ISI:000254982100022	en
dc.identifier.volume	123	en
dc.identifier.issue	1-2	en
dc.identifier.spage	166	en
dc.identifier.epage	170	en