Fermentation characteristics of Fusarium oxysporum grown on acetate

Panagiotou, G; Pachidou, F; Petroutsos, D; Olsson, L; Christakopoulos, P

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Pachidou, F	en
dc.contributor.author	Petroutsos, D	en
dc.contributor.author	Olsson, L	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:28:23Z
dc.date.available	2014-03-01T01:28:23Z
dc.date.issued	2008	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18837
dc.subject	acetate metabolism	en
dc.subject	acetate-glucose co-metabolism	en
dc.subject	extracellular metabolites	en
dc.subject	Fusarium oxysporum	en
dc.subject	intracellular metabolites	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	METABOLISM	en
dc.subject.other	CELLULOSE	en
dc.subject.other	GLUCOSE	en
dc.subject.other	ETHANOL	en
dc.title	Fermentation characteristics of Fusarium oxysporum grown on acetate	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2008.01.017	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biortech.2008.01.017	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	In this study, the growth characteristics of Fusarium oxysporum were evaluated in minimal medium using acetate or different mixtures of acetate and glucose as carbon source. The minimum inhibitory concentration (MIC) of acetic acid that F oxysporum cells could tolerate was 0.8% w/v while glucose was consumed preferentially to acetate. The activity of isocitrate lyase was high when cells were grown on acetate and acetate plus glucose indicating an activation of the glyoxylate cycle. Investigation of the metabolic fingerprinting and footprinting revealed higher levels of intracellular and extracellular TCA cycle intermediates when F. oxysporum cells were grown on mixtures of acetate and glucose compared to growth on only glucose. Our data support the hypothesis that it higher flux through TCA cycle during acetate consumption could significantly increase the pool of NADH, resulting in the activation of succinate-propionate pathway which consumes reducing power (NADH) via conversion of succinate to propionyl-CoA and produce propionate. (C) 2008 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	BIORESOURCE TECHNOLOGY	en
dc.identifier.doi	10.1016/j.biortech.2008.01.017	en
dc.identifier.isi	ISI:000257367600084	en
dc.identifier.volume	99	en
dc.identifier.issue	15	en
dc.identifier.spage	7397	en
dc.identifier.epage	7401	en