Fungal multienzyme production on industrial by-products of the citrus-processing industry

Mamma, D; Kourtoglou, E; Christakopoulos, P

dc.contributor.author	Mamma, D	en
dc.contributor.author	Kourtoglou, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:28:29Z
dc.date.available	2014-03-01T01:28:29Z
dc.date.issued	2008	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18853
dc.subject	Hydrolysis	en
dc.subject	Multienzyme production	en
dc.subject	Orange peels	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Degradation	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Optimization	en
dc.subject.other	Multienzyme production	en
dc.subject.other	Orange peels	en
dc.subject.other	Fungi	en
dc.subject.other	beta fructofuranosidase	en
dc.subject.other	cellulose	en
dc.subject.other	glucan synthase	en
dc.subject.other	hemicellulose	en
dc.subject.other	multienzyme complex	en
dc.subject.other	pectate lyase	en
dc.subject.other	pectin	en
dc.subject.other	polygalacturonase	en
dc.subject.other	xylan 1,4 beta xylosidase	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	Degradation	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	Fungi	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Optimization	en
dc.subject.other	comparative study	en
dc.subject.other	enzyme activity	en
dc.subject.other	fermentation	en
dc.subject.other	food industry	en
dc.subject.other	food processing	en
dc.subject.other	fruit	en
dc.subject.other	fungus	en
dc.subject.other	hydrolysis	en
dc.subject.other	industrial waste	en
dc.subject.other	optimization	en
dc.subject.other	pH	en
dc.subject.other	polysaccharide	en
dc.subject.other	solid waste	en
dc.subject.other	sugar	en
dc.subject.other	article	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	citrus fruit	en
dc.subject.other	controlled study	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	hydrolysis	en
dc.subject.other	microbial degradation	en
dc.subject.other	moisture	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	nonhuman	en
dc.subject.other	Penicillium	en
dc.subject.other	pH	en
dc.subject.other	priority journal	en
dc.subject.other	solid state fermentation	en
dc.subject.other	Citrus	en
dc.subject.other	Food Industry	en
dc.subject.other	Fungi	en
dc.subject.other	Multienzyme Complexes	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Citrus	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	Penicillium decumbens	en
dc.title	Fungal multienzyme production on industrial by-products of the citrus-processing industry	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2007.05.018	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biortech.2007.05.018	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Orange peels is the principal solid by-product of the citrus processing industry and the disposal of the fresh peels is becoming a major problem to many factories. Dry citrus peels are rich in pectin, cellulose and hemicellulose and may be used as a fermentation substrate. Production of multienzyme preparations containing pectinolytic, cellulolytic and xylanolytic enzymes by the mesophilic fungi Aspergillus niger BTL, Fusarium oxysporum F3, Neurospora crassa DSM 1129 and Penicillium decumbens under solid-state fermentation (SSF) on dry orange peels was enhanced by optimization of initial pH of the culture medium and initial moisture level. Under optimal conditions A. niger BTL was by far the most potent strain in polygalacturonase and pectate lyase, production followed by F. oxysporum F3, N. crassa DSM 1129 and P. decumbens. N. crassa DSM 1129 produced the highest endoglucanase activity and P. decumbens the lowest one. Comparison of xylanase production revealed that A. niger BTL produced the highest activity followed by N. crassa DSM 1129, P. decumbens and F. oxysporum F3. N. crassa DSM 1129 and P. decumbens did not produce any beta-xylosidase activity, while A. niger BTL produced approximately 10 times more P-xylosidase than F. oxysporum F3. The highest invertase activity was produced by A. niger BTL while the lowest ones by F. oxysporum F3 and P. decumbens. After SSF of the four fungi, under optimal conditions, the fermented substrate was either directly exposed to autohydrolysis or new material was added, and the in situ produced multienzyme systems were successfully used for the partial degradation of orange peels polysaccharides and the liberation of fermentable sugars. (c) 2007 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Bioresource Technology	en
dc.identifier.doi	10.1016/j.biortech.2007.05.018	en
dc.identifier.isi	ISI:000253850100040	en
dc.identifier.volume	99	en
dc.identifier.issue	7	en
dc.identifier.spage	2373	en
dc.identifier.epage	2383	en