Hydrolysis and fermentation of brewer's spent grain by Neurospora crassa

Xiros, C; Topakas, E; Katapodis, P; Christakopoulos, P

dc.contributor.author	Xiros, C	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:28:39Z
dc.date.available	2014-03-01T01:28:39Z
dc.date.issued	2008	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18891
dc.subject	Brewers spent grain	en
dc.subject	Consolidated bioprocessing	en
dc.subject	Ethanol	en
dc.subject	Pre-treatment	en
dc.subject	Solid-state culture	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Bioreactors	en
dc.subject.other	Fermentation	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Optimization	en
dc.subject.other	Brewers spent grain	en
dc.subject.other	Consolidated bioprocessing	en
dc.subject.other	Solid-state culture	en
dc.subject.other	Ethanol	en
dc.subject.other	acetylesterase	en
dc.subject.other	alcohol	en
dc.subject.other	alpha arabinofuranosidase	en
dc.subject.other	beta glucosidase	en
dc.subject.other	cellulose 1,4 beta cellobiosidase	en
dc.subject.other	esterase	en
dc.subject.other	feruloylesterase	en
dc.subject.other	glucan synthase	en
dc.subject.other	multienzyme complex	en
dc.subject.other	xylan endo 1,3 beta xylosidase	en
dc.subject.other	Bioreactors	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Optimization	en
dc.subject.other	bioreactor	en
dc.subject.other	brewing industry	en
dc.subject.other	cellulose	en
dc.subject.other	electrokinesis	en
dc.subject.other	enzyme	en
dc.subject.other	ethanol	en
dc.subject.other	experimental study	en
dc.subject.other	fermentation	en
dc.subject.other	fungus	en
dc.subject.other	hydrolysis	en
dc.subject.other	laboratory method	en
dc.subject.other	lignin	en
dc.subject.other	sodium	en
dc.subject.other	aeration	en
dc.subject.other	article	en
dc.subject.other	bioreactor	en
dc.subject.other	brewing	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme mechanism	en
dc.subject.other	food processing	en
dc.subject.other	fungus culture	en
dc.subject.other	hydrolysis	en
dc.subject.other	isoelectric focusing	en
dc.subject.other	moisture	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	solid state	en
dc.subject.other	solid state fermentation	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Furans	en
dc.subject.other	Hydrolases	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Monosaccharides	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.subject.other	Substrate Specificity	en
dc.subject.other	Sulfuric Acids	en
dc.subject.other	Fungi	en
dc.subject.other	Neurospora crassa	en
dc.title	Hydrolysis and fermentation of brewer's spent grain by Neurospora crassa	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2007.11.010	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biortech.2007.11.010	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	In this study, the ethanol production by the mesophilic fungus Neurospora crassa from BG was studied and optimized concerning the induction of lignocellulose degrading enzymes and the production phase as well. The production of cellulolytic and hemicellulolytic enzymes was studied under solid-state cultivation (SSC). SSC in a laboratory horizontal bioreactor using the optimized medium, WS and BG in the ratio 1: 1 and initial moisture level 61.51%, allowed the large scale production of the multienzymatic system. Similar yields with those from flasks experiments, as high as 1073,56,4.2,1.6,3.1,5.7 and 0.52 U g(-1) carbon source of xylanase, endoglucanase, cellobiohydrolase, beta-glucosidase, alpha-L-arabinofuranosidase, acetyl esterase and feruloyl esterase, respectively, were obtained. Chromogenic (fluorogenic) 4-methylumbelliferyl substrates were used to characterize the major activities of the multienzyme component, after the separation by isoelectric focusing (IEF) electrophoresis. Alkali pre-treated BG was used for ethanol production. A yield of about 74 g of ethanol kg(-1) dry BG (5,6 g L-1) was obtained under optimum conditions (aeration 0.1 vvm, pre-treatment with 1 g NaOH 10 g(-1) dry BG). (C) 2007 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Bioresource Technology	en
dc.identifier.doi	10.1016/j.biortech.2007.11.010	en
dc.identifier.isi	ISI:000256654600019	en
dc.identifier.volume	99	en
dc.identifier.issue	13	en
dc.identifier.spage	5427	en
dc.identifier.epage	5435	en