Preparation of multipurpose cross-linked enzyme aggregates and their application to production of alkyl ferulates

Vafiadi, C; Topakas, E; Christakopoulos, P

dc.contributor.author	Vafiadi, C	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:29:03Z
dc.date.available	2014-03-01T01:29:03Z
dc.date.issued	2008	en
dc.identifier.issn	1381-1177	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19098
dc.subject	Alkyl ferulates	en
dc.subject	CLEA	en
dc.subject	Enzyme immobilization	en
dc.subject	Feruloyl esterases	en
dc.subject	Transesterification	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	Biocatalysts	en
dc.subject.other	Crosslinking	en
dc.subject.other	Enzyme immobilization	en
dc.subject.other	Synthesis (chemical)	en
dc.subject.other	Ternary systems	en
dc.subject.other	Transesterification	en
dc.subject.other	Alkyl ferulates	en
dc.subject.other	Cross linked enzyme aggregates (CLEA)	en
dc.subject.other	Feruloyl esterases	en
dc.subject.other	Enzyme activity	en
dc.subject.other	1 butyl ferulate	en
dc.subject.other	alkyl group	en
dc.subject.other	butanol	en
dc.subject.other	depol 670l	en
dc.subject.other	depol 740l	en
dc.subject.other	esterase	en
dc.subject.other	ferulic acid	en
dc.subject.other	ferulic acid derivative	en
dc.subject.other	ferulic acid methyl ester	en
dc.subject.other	feruloyl esterase	en
dc.subject.other	fungal enzyme	en
dc.subject.other	hexane	en
dc.subject.other	immobilized enzyme	en
dc.subject.other	industrial enzyme	en
dc.subject.other	ultraflo l	en
dc.subject.other	water	en
dc.subject.other	article	en
dc.subject.other	biocatalyst	en
dc.subject.other	biotransformation	en
dc.subject.other	enzyme analysis	en
dc.subject.other	enzyme stability	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	precipitation	en
dc.subject.other	product recovery	en
dc.subject.other	protein cross linking	en
dc.subject.other	reaction optimization	en
dc.title	Preparation of multipurpose cross-linked enzyme aggregates and their application to production of alkyl ferulates	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.molcatb.2007.12.005	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.molcatb.2007.12.005	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Commercial rnulticomponent enzyme preparations, Ultraflo L, Depol 740L and Depot 670L, with feruloyl esterase activity, were tested for the transesterification of methyl ferulate to 1-butyl ferulate in their free and immobilized form using as a reaction system a ternary water-organic mixture consisting of n-hexane, 1-butanol and water. A number of factors affecting enzymes precipitation and cross-linking into cross-linked enzyme aggregates (CLEAs) have been investigated. Consecutive optimization of the precipitant type and cross-linker concentration resulted in CLEAs showing higher operational stability and synthetic activity compared to the free enzymes' forms. Under certain optimization conditions. conversion yields of 97%, 87% and 5%, were obtained by CLEAs prepared from Ultraflo L, Depol 740L and Depol 670L, respectively. The activities initially present in the three commercial preparations were completely retained after cross-linking resulting in multipurpose biocatalysts which have the potential to carry out different and independent reactions. This work is consistent to the novel CLEA concept called combi-CLEA. (C) 2007 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Molecular Catalysis B: Enzymatic	en
dc.identifier.doi	10.1016/j.molcatb.2007.12.005	en
dc.identifier.isi	ISI:000257590000006	en
dc.identifier.volume	54	en
dc.identifier.issue	1-2	en
dc.identifier.spage	35	en
dc.identifier.epage	41	en