Transcriptomic analysis of Saccharomyces cerevisiae physiology in the context of galactose assimilation perturbations

Syriopoulos, C; Panayotarou, A; Lai, K; Klapa, MI

dc.contributor.author	Syriopoulos, C	en
dc.contributor.author	Panayotarou, A	en
dc.contributor.author	Lai, K	en
dc.contributor.author	Klapa, MI	en
dc.date.accessioned	2014-03-01T01:29:24Z
dc.date.available	2014-03-01T01:29:24Z
dc.date.issued	2008	en
dc.identifier.issn	1742-206X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19252
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	galactose	en
dc.subject.other	glucose	en
dc.subject.other	article	en
dc.subject.other	comparative study	en
dc.subject.other	fungal gene	en
dc.subject.other	gene expression profiling	en
dc.subject.other	gene expression regulation	en
dc.subject.other	genetic transcription	en
dc.subject.other	genetics	en
dc.subject.other	metabolism	en
dc.subject.other	phenotype	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.subject.other	Galactose	en
dc.subject.other	Gene Expression Profiling	en
dc.subject.other	Gene Expression Regulation, Fungal	en
dc.subject.other	Genes, Fungal	en
dc.subject.other	Glucose	en
dc.subject.other	Phenotype	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.subject.other	Transcription, Genetic	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.title	Transcriptomic analysis of Saccharomyces cerevisiae physiology in the context of galactose assimilation perturbations	en
heal.type	journalArticle	en
heal.identifier.primary	10.1039/b718732g	en
heal.identifier.secondary	http://dx.doi.org/10.1039/b718732g	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Despite being extensively studied in various organisms due to scientific, industrial and medical interest, the galactose assimilation function and regulation, and especially its interaction with other parts of cellular physiology, have not been fully elucidated yet. The post-genomic era holistic techniques (""omics"") could assist towards this goal. In this paper, we holistically analyzed full-genome Saccharomyces cerevisiae transcriptional profiling data concerning its glucose- and galactose-grown wild-type (WT) physiology and its glucose-grown gal7-deficient (GAL7Δ) physiology, as these were obtained in the experiment described in Lai and Elsas (Biochem. Biophys. Res. Commun. 2000, 271, 392-400). The gal7 gene encodes for the second enzyme of the galactose assimilation, Leloir, pathway, and its deficiency in humans causes a potentially lethal disease, named ""classic galactosemia"". Analysis of the galactose-grown compared to the glucose-grown WT physiology indicated a significant increase in the transcriptional expression of the ribosomal machinery and decrease in the transcriptional activity of the fatty acids' β-oxidation at the peroxisomes. Comparison of GAL7Δ to WT physiology in glucose indicated a significant transcriptional increase in the mitochondrial activity and the rate of catabolism of disaccharides, including sucrose, mannose and trehalose, towards amplified biosynthesis of the main cell wall components. Comparison with other physiological conditions indicated strong correlation between the glucose-grown GAL7Δ transcriptional physiology and the WT growth under glucose derepression conditions. Finally, the acquired results and the large number of still unknown genes that were related to the galactose assimilation regulation indicated the need for further, specifically designed, perturbations and integrated analyses of multiple levels of cellular function. © The Royal Society of Chemistry 2008.	en
heal.publisher	ROYAL SOC CHEMISTRY	en
heal.journalName	Molecular BioSystems	en
dc.identifier.doi	10.1039/b718732g	en
dc.identifier.isi	ISI:000259038300008	en
dc.identifier.volume	4	en
dc.identifier.issue	9	en
dc.identifier.spage	937	en
dc.identifier.epage	949	en