Adsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substrates

Mamma, D; Hatzinikolaou, D; Kekos, D; Stamatis, H; Kalogeris, E

dc.contributor.author	Mamma, D	en
dc.contributor.author	Hatzinikolaou, D	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Kalogeris, E	en
dc.date.accessioned	2014-03-01T01:29:48Z
dc.date.available	2014-03-01T01:29:48Z
dc.date.issued	2009	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19347
dc.subject	Adsorption	en
dc.subject	Cellulose	en
dc.subject	Endoglucanase	en
dc.subject	Thermoascus aurantiacus	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Adsorption kinetics	en
dc.subject.other	Anion-exchange chromatographies	en
dc.subject.other	Binding capacities	en
dc.subject.other	Binding domains	en
dc.subject.other	Cellulosic materials	en
dc.subject.other	Cellulosic substrates	en
dc.subject.other	Crystalline cellulose	en
dc.subject.other	Endoglucanase	en
dc.subject.other	Enzyme productions	en
dc.subject.other	High affinities	en
dc.subject.other	In-vitro	en
dc.subject.other	Langmuir isotherms	en
dc.subject.other	Liquid mediums	en
dc.subject.other	Solid particles	en
dc.subject.other	Solid-state fermentations	en
dc.subject.other	Submerged cultures	en
dc.subject.other	Thermoascus aurantiacus	en
dc.subject.other	Thermophilic fungus	en
dc.subject.other	Adsorption	en
dc.subject.other	Biochemical engineering	en
dc.subject.other	Cellulose	en
dc.subject.other	Chromatographic analysis	en
dc.subject.other	Crystalline materials	en
dc.subject.other	Enzyme activity	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	Substrates	en
dc.subject.other	Textile processing	en
dc.subject.other	Adsorption	en
dc.subject.other	Biochemistry	en
dc.subject.other	Chromatography	en
dc.subject.other	Cultivation	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucanase	en
dc.subject.other	Substrates	en
dc.subject.other	Fungi	en
dc.subject.other	Thermoascus	en
dc.subject.other	Thermoascus aurantiacus	en
dc.title	Adsorption of major endoglucanase from Thermoascus aurantiacus on cellulosic substrates	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11274-008-9949-2	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s11274-008-9949-2	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	A thermostable endoglucanase (EndoI) was produced by the thermophilic fungus Thermoascus aurantiacus when grown on cellulosic materials under submerged culture (SC) and solid-state fermentation (SSF). In both cultivation techniques a considerable amount of enzyme activity remained adsorbed onto solid particles, and this was taken into consideration when modeling enzyme production. The results were compatible with the assumption that, following its synthesis, an amount of EndoI was bound on substrate and gradually released into the liquid medium. Adsorption of the enzyme on crystalline cellulose was confirmed in vitro by experiments with purified endoglucanase, which was isolated by anion exchange chromatography. The Langmuir isotherm could efficiently describe the adsorption kinetics, and the estimated A max and K ad values compared with those obtained for cellulases bearing a binding domain. EndoI displayed high affinity for crystalline cellulose and low binding capacity, which could be beneficial in textile processing. © 2009 Springer Science+Business Media B.V.	en
heal.publisher	SPRINGER	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s11274-008-9949-2	en
dc.identifier.isi	ISI:000264970200006	en
dc.identifier.volume	25	en
dc.identifier.issue	5	en
dc.identifier.spage	781	en
dc.identifier.epage	788	en