Chemical binding of biomolecules to micropatterned epoxy modified surfaces for biosensing applications

Pavli, P; Petrou, PS; Niakoula, D; Douvas, AM; Chatzichristidi, M; Kakabakos, SE; Dimotikali, D; Argitis, P

dc.contributor.author	Pavli, P	en
dc.contributor.author	Petrou, PS	en
dc.contributor.author	Niakoula, D	en
dc.contributor.author	Douvas, AM	en
dc.contributor.author	Chatzichristidi, M	en
dc.contributor.author	Kakabakos, SE	en
dc.contributor.author	Dimotikali, D	en
dc.contributor.author	Argitis, P	en
dc.date.accessioned	2014-03-01T01:29:59Z
dc.date.available	2014-03-01T01:29:59Z
dc.date.issued	2009	en
dc.identifier.issn	0167-9317	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19429
dc.subject	Biotin derivatives	en
dc.subject	Chemically amplified photoresist	en
dc.subject	Epoxy groups	en
dc.subject	Photolithography	en
dc.subject	Protein patterning through covalent bonds	en
dc.subject.classification	Engineering, Electrical & Electronic	en
dc.subject.classification	Nanoscience & Nanotechnology	en
dc.subject.classification	Optics	en
dc.subject.classification	Physics, Applied	en
dc.subject.other	Amine groups	en
dc.subject.other	Biosensing applications	en
dc.subject.other	Biosensing devices	en
dc.subject.other	Biotin derivatives	en
dc.subject.other	Chemical bindings	en
dc.subject.other	Chemically amplified photoresist	en
dc.subject.other	Covalent bondings	en
dc.subject.other	Epoxy groups	en
dc.subject.other	Functionalized	en
dc.subject.other	High throughputs	en
dc.subject.other	Lithographic process	en
dc.subject.other	Micro-patterned	en
dc.subject.other	Micro/nanostructures	en
dc.subject.other	Modified surfaces	en
dc.subject.other	Multi-analyte	en
dc.subject.other	Patterned surfaces	en
dc.subject.other	Protein patterning through covalent bonds	en
dc.subject.other	Succinimidyl esters	en
dc.subject.other	Amines	en
dc.subject.other	Biomolecules	en
dc.subject.other	Biosensors	en
dc.subject.other	Coenzymes	en
dc.subject.other	Esters	en
dc.subject.other	Photoresistors	en
dc.subject.other	Photoresists	en
dc.subject.other	Surface treatment	en
dc.subject.other	Derivatives	en
dc.title	Chemical binding of biomolecules to micropatterned epoxy modified surfaces for biosensing applications	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.mee.2008.11.009	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.mee.2008.11.009	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	The formation of micro/nanostructures that can be used for binding of specific biomolecules is of great importance for the development of multi-analyte biosensing devices and high-throughput microarrays. In this paper, we report on the chemical binding of biotin on an epoxy photolithographically patterned surface. In particular, we used a negative chemically amplified epoxy resist (EPR) in order to create very small structures by photolithography, in which biotin derivatives functionalized with succinimidyl ester or an amine group were immobilized through covalent bonding. Lithographic process with exposure at 248 nm was optimized, and structures with diameter down to 0.5 mu m were demonstrated, where biomolecules were successfully bound. (C) 2008 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Microelectronic Engineering	en
dc.identifier.doi	10.1016/j.mee.2008.11.009	en
dc.identifier.isi	ISI:000267273300269	en
dc.identifier.volume	86	en
dc.identifier.issue	4-6	en
dc.identifier.spage	1473	en
dc.identifier.epage	1476	en